Epithelial-specific keratin gene expression: Identification of a 300 base-pair controlling segment

C. K. Jiang, H. S. Epstein, Marjana Tomic-Canic, I. M. Freedberg, M. Blumenberg

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To elucidate the elements required for regulation of keratin expression in epidermis, we have linked a short, 300 base pair segment, corresponding to the promoter region of a human K # 14 gene, to the chloramphenicol-acetyl-transferase gene. This construct was introduced into various mammalian cell lines and primary cultures via Ca3(PO4)2 precipitation. The 300 base pair segment from the keratin gene promoter region was active in all epithelial cells studied including transformed, simple epithelial cells such as HeLa and ME-180, cell lines derived from stratified epithelium, such as SCC-12, as well as primary cultures of epithelial cells. The construct was inactive in all non-epithelial cells tested including fibroblasts and melanocytes. The segment does not function as a silencer in nonepithelial cells but it can function as an enhancer in epithelial cells. Using the polymerase chain reaction we have constructed a series of deletions of the promoter and have localized an essential function within a 40 bp sequence. We conclude that we have identified the keratin gene promoter that is sufficient to confer epithelial-specific expression.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalNucleic Acids Research
Volume18
Issue number2
StatePublished - Jan 25 1990
Externally publishedYes

Fingerprint

Keratin
Keratins
Gene expression
Base Pairing
Gene Expression
Genes
Epithelial Cells
Cell
Cells
Genetic Promoter Regions
Promoter
Gene
Cell Line
Melanocytes
Polymerase chain reaction
Chloramphenicol
Fibroblasts
Transferases
Cell culture
Epidermis

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Jiang, C. K., Epstein, H. S., Tomic-Canic, M., Freedberg, I. M., & Blumenberg, M. (1990). Epithelial-specific keratin gene expression: Identification of a 300 base-pair controlling segment. Nucleic Acids Research, 18(2), 247-253.

Epithelial-specific keratin gene expression : Identification of a 300 base-pair controlling segment. / Jiang, C. K.; Epstein, H. S.; Tomic-Canic, Marjana; Freedberg, I. M.; Blumenberg, M.

In: Nucleic Acids Research, Vol. 18, No. 2, 25.01.1990, p. 247-253.

Research output: Contribution to journalArticle

Jiang, CK, Epstein, HS, Tomic-Canic, M, Freedberg, IM & Blumenberg, M 1990, 'Epithelial-specific keratin gene expression: Identification of a 300 base-pair controlling segment', Nucleic Acids Research, vol. 18, no. 2, pp. 247-253.
Jiang, C. K. ; Epstein, H. S. ; Tomic-Canic, Marjana ; Freedberg, I. M. ; Blumenberg, M. / Epithelial-specific keratin gene expression : Identification of a 300 base-pair controlling segment. In: Nucleic Acids Research. 1990 ; Vol. 18, No. 2. pp. 247-253.
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