TY - JOUR
T1 - Episomal and nonepisomal herpesvirus DNA in lymphoid tumor cell lines.
AU - Kaschka-Dierich, C.
AU - Bauer, I.
AU - Fleckenstein, B.
AU - Desrosiers, R. C.
PY - 1981
Y1 - 1981
N2 - Tumor cell lines derived from Herpesvirus saimiri (H. saimiri)- and Herpesvirus ateles (H. ateles)-induced lymphomas of New World primates and rabbits contain multiple copies of viral genomes. Partial denaturation mapping and blot hybridizations of episomal DNA from lymphoid tumor cell line No. 1670 showed that a 12.5md-fragment is missing which represents the EcoRI D- and H-fragments of virion L-DNA. However, the missing piece can be demonstrated in total cellular DNA by reassociation kinetics, possibly because it persists in integrated form. Both episomal and nonepisomal H-DNA are heavily methylated in a number of the lymphoid cell lines, and methylation may be reduced by conventional methylation inhibitors (S-adenosyl homocystein, SIBA) as well as by the tumor promoting phorbol ester TPA.
AB - Tumor cell lines derived from Herpesvirus saimiri (H. saimiri)- and Herpesvirus ateles (H. ateles)-induced lymphomas of New World primates and rabbits contain multiple copies of viral genomes. Partial denaturation mapping and blot hybridizations of episomal DNA from lymphoid tumor cell line No. 1670 showed that a 12.5md-fragment is missing which represents the EcoRI D- and H-fragments of virion L-DNA. However, the missing piece can be demonstrated in total cellular DNA by reassociation kinetics, possibly because it persists in integrated form. Both episomal and nonepisomal H-DNA are heavily methylated in a number of the lymphoid cell lines, and methylation may be reduced by conventional methylation inhibitors (S-adenosyl homocystein, SIBA) as well as by the tumor promoting phorbol ester TPA.
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U2 - 10.1007/978-3-642-67984-1_32
DO - 10.1007/978-3-642-67984-1_32
M3 - Article
C2 - 6274746
AN - SCOPUS:0019764320
VL - 26
SP - 197
EP - 203
JO - Haematology and blood transfusion
JF - Haematology and blood transfusion
SN - 0171-7111
ER -