Episodic activation of the rat GnRH promoter

Role of the homeoprotein Oct-1

Rafael Vazquez-Martinez, Gilles M. Leclerc, Margaret E. Wierman, Fredric R. Boockfor

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recent reports demonstrate that the rat GnRH promoter is activated in an episodic fashion in immortalized GnRH neurons, but little information is available on molecular processes that contribute to this phenomenon. In this study, we dissected the regions of the rat GnRH promoter that mediate these effects by testing a series of 5′ deletion luciferase reporter constructs on the pattern of photonic emissions from single, living GT1-7 GnRH neuronal cells. Deletion analysis revealed that the region -2012/-1597 that contains the neuron-specific enhancer (NSE) was required for the elaboration of pulses of GnRH promoter activity. The importance of this region was supported by observations that episodic reporter activity could be transferred to a neutral nonpulsatile promoter (Rous sarcoma virus, RSV180). Immunoneutralization of Oct-1 as well as mutation of an octamer binding site located at -1787/-1783 (AT-a site) blocked the pulsatile GnRH promoter activity in GT1-7 neuronal cells. Taken together, our findings indicate that episodic GnRH gene expression is a promoter-dependent phenomenon, which is mediated by Oct-1 interaction with regulatory elements in the NSE region.

Original languageEnglish
Pages (from-to)2093-2100
Number of pages8
JournalMolecular Endocrinology
Volume16
Issue number9
DOIs
StatePublished - Sep 1 2002
Externally publishedYes

Fingerprint

Homeodomain Proteins
Gonadotropin-Releasing Hormone
Neurons
Optics and Photonics
Rous sarcoma virus
Luciferases
Binding Sites
Gene Expression
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Vazquez-Martinez, R., Leclerc, G. M., Wierman, M. E., & Boockfor, F. R. (2002). Episodic activation of the rat GnRH promoter: Role of the homeoprotein Oct-1. Molecular Endocrinology, 16(9), 2093-2100. https://doi.org/10.1210/me.2002-0139

Episodic activation of the rat GnRH promoter : Role of the homeoprotein Oct-1. / Vazquez-Martinez, Rafael; Leclerc, Gilles M.; Wierman, Margaret E.; Boockfor, Fredric R.

In: Molecular Endocrinology, Vol. 16, No. 9, 01.09.2002, p. 2093-2100.

Research output: Contribution to journalArticle

Vazquez-Martinez, R, Leclerc, GM, Wierman, ME & Boockfor, FR 2002, 'Episodic activation of the rat GnRH promoter: Role of the homeoprotein Oct-1', Molecular Endocrinology, vol. 16, no. 9, pp. 2093-2100. https://doi.org/10.1210/me.2002-0139
Vazquez-Martinez, Rafael ; Leclerc, Gilles M. ; Wierman, Margaret E. ; Boockfor, Fredric R. / Episodic activation of the rat GnRH promoter : Role of the homeoprotein Oct-1. In: Molecular Endocrinology. 2002 ; Vol. 16, No. 9. pp. 2093-2100.
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