Epigallocatechin-gallate stimulates NF-E2-related factor and heme oxygenase-1 via caveolin-1 displacement

Yuanyuan Zheng, Andrew Morris, Manjula Sunkara, Joseph Layne, Michal J Toborek, Bernhard Hennig

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Flavonoids, such as the tea catechin epigallocatechin-gallate (EGCG), can protect against atherosclerosis by decreasing vascular endothelial cell inflammation. Heme oxygenase-1 (HO-1) is an enzyme that plays an important role in vascular physiology, and its induction may provide protection against atherosclerosis. Heme oxygenase-1 can be compartmentalized in caveolae in endothelial cells. Caveolae are plasma microdomains important in vesicular transport and the regulation of signaling pathways associated with the pathology of vascular diseases. We hypothesize that caveolae play a role in the uptake and transport of EGCG and mechanisms associated with the anti-inflammatory properties of this flavonoid. To test this hypothesis, we explored the effect of EGCG on the induction of NF-E2-related factor (Nrf2) and HO-1 in endothelial cells with or without functional caveolae. Treatment with EGCG activated Nrf2 and increased HO-1 expression and cellular production of bilirubin. In addition, EGCG rapidly accumulated in caveolae, which was associated with caveolin-1 displacement from the plasma membrane towards the cytosol. Similar to EGCG treatment, silencing of caveolin-1 by siRNA technique also resulted in up-regulation of Nrf2, HO-1 and bilirubin production. These data suggest that EGCG-induced caveolin-1 displacement may reduce endothelial inflammation.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalJournal of Nutritional Biochemistry
Volume23
Issue number2
DOIs
StatePublished - Feb 1 2012
Externally publishedYes

Fingerprint

Caveolin 1
Heme Oxygenase-1
Caveolae
Endothelial cells
Endothelial Cells
Bilirubin
Flavonoids
Atherosclerosis
Inflammation
Catechin
Physiology
Pathology
Tea
Cell membranes
epigallocatechin gallate
Vascular Diseases
Cytosol
Small Interfering RNA
Blood Vessels
Anti-Inflammatory Agents

Keywords

  • Atherosclerosis
  • Caveolae
  • EGCG
  • Endothelial cells
  • Nrf2

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Epigallocatechin-gallate stimulates NF-E2-related factor and heme oxygenase-1 via caveolin-1 displacement. / Zheng, Yuanyuan; Morris, Andrew; Sunkara, Manjula; Layne, Joseph; Toborek, Michal J; Hennig, Bernhard.

In: Journal of Nutritional Biochemistry, Vol. 23, No. 2, 01.02.2012, p. 163-168.

Research output: Contribution to journalArticle

Zheng, Yuanyuan ; Morris, Andrew ; Sunkara, Manjula ; Layne, Joseph ; Toborek, Michal J ; Hennig, Bernhard. / Epigallocatechin-gallate stimulates NF-E2-related factor and heme oxygenase-1 via caveolin-1 displacement. In: Journal of Nutritional Biochemistry. 2012 ; Vol. 23, No. 2. pp. 163-168.
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