Epidermal growth factor receptor gene expression in estrogen receptor-positive and negative human breast cancer cell lines.

N. E. Davidson, E. P. Gelmann, Marc E Lippman, R. B. Dickson

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Expression of epidermal growth factor (EGF) receptor by human breast cancer tissues has an inverse relationship with expression of the estrogen receptor and may be associated with a poor clinical response. We have studied the regulation of EGF receptor expression in a series of human breast cancer cell lines with varying degrees of estrogen responsiveness. Three estrogen receptor-positive lines, MCF-7, ZR-75-1, and T47D, were found to have less than 70,000 EGF binding sites per cell by radioreceptor assay and were growth stimulated in vitro by EGF. Four estrogen receptor-negative lines, MDA-MB-231, Hs578T, EVSA-T, and BT-20, contained greater than 70,000 EGF binding sites per cell and showed no in vitro growth stimulation by EGF. In all cell lines EGF receptor number was correlated with the amount of EGF receptor protein and RNA. Differences in EGF receptor expression between the cell types was not due to amplification of the EGF receptor gene. Rather, variations in EGF receptor expression between lines were due, at least in part, to differences in the rate of EGF gene transcription as determined by nuclear run-off studies. Our data confirm the previously described inverse relationship between expression of EGF and estrogen receptors. We show here that the absence of estrogen receptor expression in human breast cancer cell lines is associated with higher levels of functional EGF receptor protein and mRNA.

Original languageEnglish
Pages (from-to)216-223
Number of pages8
JournalMolecular endocrinology (Baltimore, Md.)
Volume1
Issue number3
StatePublished - Mar 1 1987
Externally publishedYes

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erbB-1 Genes
Epidermal Growth Factor Receptor
Estrogen Receptors
Epidermal Growth Factor
Breast Neoplasms
Gene Expression
Cell Line
Estrogens
Binding Sites
Radioligand Assay
Growth
Proteins
RNA
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Epidermal growth factor receptor gene expression in estrogen receptor-positive and negative human breast cancer cell lines. / Davidson, N. E.; Gelmann, E. P.; Lippman, Marc E; Dickson, R. B.

In: Molecular endocrinology (Baltimore, Md.), Vol. 1, No. 3, 01.03.1987, p. 216-223.

Research output: Contribution to journalArticle

Davidson, N. E. ; Gelmann, E. P. ; Lippman, Marc E ; Dickson, R. B. / Epidermal growth factor receptor gene expression in estrogen receptor-positive and negative human breast cancer cell lines. In: Molecular endocrinology (Baltimore, Md.). 1987 ; Vol. 1, No. 3. pp. 216-223.
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