Epidermal growth factor receptor activation by epidermal growth factor mediates oxidant-induced goblet cell metaplasia in human airway epithelium

S. Marina Casalino-Matsuda, Maria E. Monzon, Rosanna M. Forteza

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Mucus overproduction in inflammatory and obstructive airway diseases is associated with goblet cell (GC) metaplasia in airways. Although the mechanisms involved in GC metaplasia and mucus hypersecretion are not completely understood, association with oxidative stress and epidermal growth factor receptor (EGFR) signaling has been reported. To explore the mechanisms involved in oxidative stress-induced GC metaplasia, cultures of differentiated normal human bronchial epithelial cells grown at the air-liquid interface were exposed to reactive oxygen species (ROS) generated by xanthine/xanthine oxidase. EGFR activation and signaling was assessed by measuring EGF and transforming growth factor-α release and EGFR and 44/42MAPK phosphorylation. The GC population was evaluated by confocal microscopy. ROS-induced EGFR activation resulted in GC proliferation and increased MUC5AC gene and protein expression. Signaling was due to pro-EGF processing by tissue kallikrein (TK), which was activated by ROS-induced hyaluronan breakdown. It was inhibited by catalase, a TK inhibitor, and EGF-blocking antibodies. Exposure to recombinant TK mimicked the ROS effects, increasing the expression of MUC5AC and lactoperoxidase. In addition, ROS induced the antiapoptotic factor Bcl-2 in a TK-dependent fashion. In conclusion, ROS-induced GC metaplasia in normal human bronchial epithelial cells is associated with HA depolymerization and EGF processing by TK followed by EGFR signaling, suggesting that increases in TK activity could contribute to GC metaplasia and mucus hypersecretion in diseases such as asthma and chronic bronchitis. The data also suggest that increases in GC population could be sustained by the associated upregulation of Bcl-2 in airway epithelial cells.

Original languageEnglish
Pages (from-to)581-591
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume34
Issue number5
DOIs
StatePublished - May 1 2006
Externally publishedYes

Fingerprint

Tissue Kallikreins
Goblet Cells
Metaplasia
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Oxidants
Reactive Oxygen Species
Epithelium
Chemical activation
Oxidative stress
Mucus
Epithelial Cells
Lactoperoxidase
Depolymerization
Phosphorylation
Oxidative Stress
Blocking Antibodies
Xanthine
Xanthine Oxidase
Confocal microscopy

Keywords

  • EGFR
  • Goblet cells
  • Hyaluronan
  • NHBE cells
  • Tissue kallikrein

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Epidermal growth factor receptor activation by epidermal growth factor mediates oxidant-induced goblet cell metaplasia in human airway epithelium. / Casalino-Matsuda, S. Marina; Monzon, Maria E.; Forteza, Rosanna M.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 34, No. 5, 01.05.2006, p. 581-591.

Research output: Contribution to journalArticle

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