Abstract
Methods for the microdetermination of glycogen and amylopectin and their average unit-chain lengths are described. The determination of the polysaccharides relies on their quantitative conversion into glucose by amyloglucosidase. The chainlength determination requires the simultaneous use of two enzymes, pullulanase and β-amylase, for the complete degradation of the polysaccharides to maltose and glucose. The specific measurement of the glucose formed permits the calculation of chain length. The methods were tested with samples of glycogen and amylopectin, whose chain lengths had been determined by periodate oxidation. In all cases there was good agreement between the results of the chemical and enzymic determinations. The analyses may be performed with less than 1 mg of polysaccharide, and so are particularly appropriate to the examination of glycogen in biopsy specimens.
| Original language | English |
|---|---|
| Pages (from-to) | 162-167 |
| Number of pages | 6 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 116 |
| Issue number | C |
| State | Published - Dec 1 1966 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
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Enzymic methods for the microdetermination of glycogen and amylopectin, and their unit-chain lengths. / Lee, E. Y C; Whelan, William J.
In: Archives of Biochemistry and Biophysics, Vol. 116, No. C, 01.12.1966, p. 162-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enzymic methods for the microdetermination of glycogen and amylopectin, and their unit-chain lengths
AU - Lee, E. Y C
AU - Whelan, William J.
PY - 1966/12/1
Y1 - 1966/12/1
N2 - Methods for the microdetermination of glycogen and amylopectin and their average unit-chain lengths are described. The determination of the polysaccharides relies on their quantitative conversion into glucose by amyloglucosidase. The chainlength determination requires the simultaneous use of two enzymes, pullulanase and β-amylase, for the complete degradation of the polysaccharides to maltose and glucose. The specific measurement of the glucose formed permits the calculation of chain length. The methods were tested with samples of glycogen and amylopectin, whose chain lengths had been determined by periodate oxidation. In all cases there was good agreement between the results of the chemical and enzymic determinations. The analyses may be performed with less than 1 mg of polysaccharide, and so are particularly appropriate to the examination of glycogen in biopsy specimens.
AB - Methods for the microdetermination of glycogen and amylopectin and their average unit-chain lengths are described. The determination of the polysaccharides relies on their quantitative conversion into glucose by amyloglucosidase. The chainlength determination requires the simultaneous use of two enzymes, pullulanase and β-amylase, for the complete degradation of the polysaccharides to maltose and glucose. The specific measurement of the glucose formed permits the calculation of chain length. The methods were tested with samples of glycogen and amylopectin, whose chain lengths had been determined by periodate oxidation. In all cases there was good agreement between the results of the chemical and enzymic determinations. The analyses may be performed with less than 1 mg of polysaccharide, and so are particularly appropriate to the examination of glycogen in biopsy specimens.
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UR - http://www.scopus.com/inward/citedby.url?scp=0014028907&partnerID=8YFLogxK
M3 - Article
C2 - 5961834
AN - SCOPUS:0014028907
VL - 116
SP - 162
EP - 167
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - C
ER -