Enzymic methods for the microdetermination of glycogen and amylopectin, and their unit-chain lengths

E. Y.C. Lee; W. J. Whelan

doi:10.1016/0003-9861(66)90024-5

Enzymic methods for the microdetermination of glycogen and amylopectin, and their unit-chain lengths

E. Y.C. Lee, W. J. Whelan

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Abstract

Methods for the microdetermination of glycogen and amylopectin and their average unit-chain lengths are described. The determination of the polysaccharides relies on their quantitative conversion into glucose by amyloglucosidase. The chainlength determination requires the simultaneous use of two enzymes, pullulanase and β-amylase, for the complete degradation of the polysaccharides to maltose and glucose. The specific measurement of the glucose formed permits the calculation of chain length. The methods were tested with samples of glycogen and amylopectin, whose chain lengths had been determined by periodate oxidation. In all cases there was good agreement between the results of the chemical and enzymic determinations. The analyses may be performed with less than 1 mg of polysaccharide, and so are particularly appropriate to the examination of glycogen in biopsy specimens.

Original language	English (US)
Pages (from-to)	162-167
Number of pages	6
Journal	Archives of Biochemistry and Biophysics
Volume	116
Issue number	C
DOIs	https://doi.org/10.1016/0003-9861(66)90024-5
State	Published - 1966
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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title = "Enzymic methods for the microdetermination of glycogen and amylopectin, and their unit-chain lengths",

abstract = "Methods for the microdetermination of glycogen and amylopectin and their average unit-chain lengths are described. The determination of the polysaccharides relies on their quantitative conversion into glucose by amyloglucosidase. The chainlength determination requires the simultaneous use of two enzymes, pullulanase and β-amylase, for the complete degradation of the polysaccharides to maltose and glucose. The specific measurement of the glucose formed permits the calculation of chain length. The methods were tested with samples of glycogen and amylopectin, whose chain lengths had been determined by periodate oxidation. In all cases there was good agreement between the results of the chemical and enzymic determinations. The analyses may be performed with less than 1 mg of polysaccharide, and so are particularly appropriate to the examination of glycogen in biopsy specimens.",

author = "Lee, {E. Y.C.} and Whelan, {W. J.}",

note = "Funding Information: It is a privilege and pleasure to take part in this collective tribute to Professor Luis F. Leloir, frum whose fundamental discoveries have stemmed the modern concepts of oligo-and poly-saccharid biosynthesis. This work was supported by a PL-480 grant of the U. S. Department of Agriculture. We thank Professor S. Sherlock and Dr. A. D. Patrick for the gift of biopsy samples, Dr. W. F. J. Cuth-bertson for the gift of amyloglucosidase, and Imperial Chemical Industries Ltd. for t,he loan of apparatus.",

year = "1966",

doi = "10.1016/0003-9861(66)90024-5",

language = "English (US)",

volume = "116",

pages = "162--167",

journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

publisher = "Academic Press Inc.",

number = "C",

}

TY - JOUR

T1 - Enzymic methods for the microdetermination of glycogen and amylopectin, and their unit-chain lengths

AU - Lee, E. Y.C.

AU - Whelan, W. J.

N1 - Funding Information: It is a privilege and pleasure to take part in this collective tribute to Professor Luis F. Leloir, frum whose fundamental discoveries have stemmed the modern concepts of oligo-and poly-saccharid biosynthesis. This work was supported by a PL-480 grant of the U. S. Department of Agriculture. We thank Professor S. Sherlock and Dr. A. D. Patrick for the gift of biopsy samples, Dr. W. F. J. Cuth-bertson for the gift of amyloglucosidase, and Imperial Chemical Industries Ltd. for t,he loan of apparatus.

PY - 1966

Y1 - 1966

N2 - Methods for the microdetermination of glycogen and amylopectin and their average unit-chain lengths are described. The determination of the polysaccharides relies on their quantitative conversion into glucose by amyloglucosidase. The chainlength determination requires the simultaneous use of two enzymes, pullulanase and β-amylase, for the complete degradation of the polysaccharides to maltose and glucose. The specific measurement of the glucose formed permits the calculation of chain length. The methods were tested with samples of glycogen and amylopectin, whose chain lengths had been determined by periodate oxidation. In all cases there was good agreement between the results of the chemical and enzymic determinations. The analyses may be performed with less than 1 mg of polysaccharide, and so are particularly appropriate to the examination of glycogen in biopsy specimens.

AB - Methods for the microdetermination of glycogen and amylopectin and their average unit-chain lengths are described. The determination of the polysaccharides relies on their quantitative conversion into glucose by amyloglucosidase. The chainlength determination requires the simultaneous use of two enzymes, pullulanase and β-amylase, for the complete degradation of the polysaccharides to maltose and glucose. The specific measurement of the glucose formed permits the calculation of chain length. The methods were tested with samples of glycogen and amylopectin, whose chain lengths had been determined by periodate oxidation. In all cases there was good agreement between the results of the chemical and enzymic determinations. The analyses may be performed with less than 1 mg of polysaccharide, and so are particularly appropriate to the examination of glycogen in biopsy specimens.

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VL - 116

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JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - C

ER -

Enzymic methods for the microdetermination of glycogen and amylopectin, and their unit-chain lengths

Abstract

ASJC Scopus subject areas

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