Enzymatic release of microspheroids containing hydroxyapatite crystals from synovium and of calcium pyrophosphate dihydrate crystals from cartilage

P. B. Halverson, H. S. Cheung, D. J. McCarty

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Incubation of minced, focally calcified, chondromatosis tissue obtained at operation from a patient with Milwaukee shoulder (rotator cuff defect and glenohumeral osteoarthritis associated with synovial fluid, hydroxyapatitie crystals in microspheroidal masses, collagenase, and neutral protease) with partially purifed mammalian synovial cell collagenase released masses of hydroxyapatite crystals of the same size as those originally found in the patient's synovial fluid. Incubation of mineral articular cartilage obtained from a shoulder joint at arthroplasty for a destructive anthropathy in a patient with generalised calcium pyrophosphate dihydrate (CPPD) crystal deposition with partially purified synovial cell collagenase freed CPPD crystals from their matrix. These data are compatible with a previously postulated mechanism linking microcrystals to destructive arthropathies, that is, crystal endocytosis by synovial cells stimulating collagenase secretion with subsequent enzymatic crystal 'strip-mining', releasing additional crystals into the synovial fluid in a self-perpetuating cycle.

Original languageEnglish (US)
Pages (from-to)527-531
Number of pages5
JournalAnnals of the Rheumatic Diseases
Volume41
Issue number5
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Enzymatic release of microspheroids containing hydroxyapatite crystals from synovium and of calcium pyrophosphate dihydrate crystals from cartilage'. Together they form a unique fingerprint.

Cite this