Enzymatic recycling of NADPH at high temperature utilizing a thermostable glucose-6-phosphate dehydrogenase from Bacillus stearothermophilus

Ramesh B. Iyer, Leonidas G. Bachas

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A high temperature NADPH recycling system was designed utilizing a thermostable glucose-6-phosphate dehydrogenase (tG6PDH) from Bacillus stearothermophilus with glucose-6-sulfate (G6S) as the substrate. The thermostable alcohol dehydrogenase (tADH) from Thermoanaerobacter (formerly Thermoanaerobium) brockii was employed to catalyze the reduction of 2-butanone to 2-butanol at 55°C. It was established that the B. stearothermophilus G6PDH is capable of utilizing G6S as the substrate (KM=50mM at 55°C). NADPH recycling based on the G6S/tG6PDH system performed optimally with a 3:1 ratio of tG6PDH:tADH, and with 1mM NADP. The system produced 75mM 2-butanol and retained 60% of NADPH after 5h at 55°C. Under the same conditions, the system using glucose-6-phosphate (G6P), the natural substrate for tG6PDH, produced only 25mM 2-butanol and retained negligible amount of the co-factor.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of Molecular Catalysis B: Enzymatic
Volume28
Issue number1
DOIs
StatePublished - Apr 2 2004
Externally publishedYes

Keywords

  • Alcohol dehydrogenase
  • Biotransformation
  • Glucose-6-phosphate dehydrogenase
  • NADPH recycling
  • Thermostable

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

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