Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension

Jason C. Schense, Jocelyne Bloch, Patrick Aebischer, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

Fibrin plays an important role in wound healing and regeneration, and enjoys widespread use in surgery and tissue engineering. The enzymatic activity of Factor XIIIa was employed to covalently incorporate exogenous bioactive peptides within fibrin during coagulation. Fibrin gels were formed with incorporated peptides from laminin and N-cadherin alone and in combination at concentrations up to 8.2 mol peptide per mole of fibrinogen. Neurite extension in vitro was enhanced when gels were augmented with exogenous peptide, with the maximal improvement reaching 75%. When this particular fibrin derivative was evaluated in rats in the repair of the severed dorsal root within polymeric tubes, the number of regenerated axons was enhanced by 85% relative to animals treated with tubes filled with unmodified fibrin. These results demonstrate that it is possible to enhance the biological activity of fibrin by enzymatically incorporating exogenous oligopeptide domains of morphoregulatory proteins.

Original languageEnglish
Pages (from-to)415-419
Number of pages5
JournalNature Biotechnology
Volume18
Issue number4
DOIs
StatePublished - May 3 2000
Externally publishedYes

Fingerprint

Neurites
Fibrin
Peptides
Gels
Bioactivity
Coagulation
Tissue engineering
Factor XIIIa
Surgery
Rats
Oligopeptides
Animals
Repair
Spinal Nerve Roots
Laminin
Cadherins
Tissue Engineering
Derivatives
Proteins
Wound Healing

Keywords

  • Factor XIII
  • Fibrin
  • Laminin
  • N-cadherin
  • Nerve regeneration

ASJC Scopus subject areas

  • Microbiology

Cite this

Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension. / Schense, Jason C.; Bloch, Jocelyne; Aebischer, Patrick; Hubbell, Jeffrey A.

In: Nature Biotechnology, Vol. 18, No. 4, 03.05.2000, p. 415-419.

Research output: Contribution to journalArticle

Schense, Jason C. ; Bloch, Jocelyne ; Aebischer, Patrick ; Hubbell, Jeffrey A. / Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension. In: Nature Biotechnology. 2000 ; Vol. 18, No. 4. pp. 415-419.
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