Enzymatic generation of hydrogen peroxide and gluconic acid chelate for chloro-organic destruction by modified Fenton reaction

Deepak Ahuja, Leonidas G Bachas, D. Bhattacharyya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Various remediation technologies are being developed for the destruction of groundwater pollutants like 2,4,6-trichlorophenol (TCP) and polychlorinated biphenyls (PCBs). This paper is focused on the oxidative dechlorination of TCP by chelate-based Fenton reaction near neutral pH using enzymatically generated hydrogen peroxide and chelate gluconic acid. For the application of Fenton reaction in in-situ remediation, its successful operation near neutral pH is essential. Hence, Fenton reaction is carried out in the presence of chelates that sequester ferrous and ferric ions, making its operation near neutral pH possible. The on-site simultaneous production of hydrogen peroxide and a non-toxic chelate for in-situ remediation of toxic organics by Fenton reaction is highly desirable. For this purpose, well-known enzyme glucose oxidase (GOx), and inexpensive reactants such as glucose and air were used. The study involved developing the correct protocols for the simultaneous enzymatic generation of hydrogen peroxide and chelate gluconic acid for the dechlorination of chlorinated organics by Fenton reaction. The oxidation of glucose was carried out using the enzyme in free and immobilized forms. The rate of production of hydrogen peroxide was determined for the homogeneous and heterogeneous systems, and was used to estimate the time required for complete consumption of glucose during the process, thus avoiding any traces of glucose in the Fenton reaction. The Fenton reaction was carried out at varying ratios of gluconic acid:Fe and under different pH conditions, and their effect on the decomposition of TCP and H 2O 2 was studied. The reaction rate was modeled in terms of the oxidation of parent compound TCP and decomposition of H 2O 2.

Original languageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages8225-8230
Number of pages6
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

Fingerprint

Hydrogen peroxide
Glucose
Remediation
Dechlorination
Acids
Enzymes
Decomposition
Oxidation
Glucose oxidase
Polychlorinated biphenyls
Reaction rates
Groundwater
Ions
Air

Keywords

  • Chelate
  • Enzyme glucose oxidase
  • Fenton reaction
  • Gluconic acid
  • Hydrogen peroxide
  • Immobilization
  • pH

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Ahuja, D., Bachas, L. G., & Bhattacharyya, D. (2005). Enzymatic generation of hydrogen peroxide and gluconic acid chelate for chloro-organic destruction by modified Fenton reaction. In AIChE Annual Meeting, Conference Proceedings (pp. 8225-8230)

Enzymatic generation of hydrogen peroxide and gluconic acid chelate for chloro-organic destruction by modified Fenton reaction. / Ahuja, Deepak; Bachas, Leonidas G; Bhattacharyya, D.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 8225-8230.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ahuja, D, Bachas, LG & Bhattacharyya, D 2005, Enzymatic generation of hydrogen peroxide and gluconic acid chelate for chloro-organic destruction by modified Fenton reaction. in AIChE Annual Meeting, Conference Proceedings. pp. 8225-8230, 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States, 10/30/05.
Ahuja D, Bachas LG, Bhattacharyya D. Enzymatic generation of hydrogen peroxide and gluconic acid chelate for chloro-organic destruction by modified Fenton reaction. In AIChE Annual Meeting, Conference Proceedings. 2005. p. 8225-8230
Ahuja, Deepak ; Bachas, Leonidas G ; Bhattacharyya, D. / Enzymatic generation of hydrogen peroxide and gluconic acid chelate for chloro-organic destruction by modified Fenton reaction. AIChE Annual Meeting, Conference Proceedings. 2005. pp. 8225-8230
@inproceedings{ae7f11a95f61464a98f356e634c0a555,
title = "Enzymatic generation of hydrogen peroxide and gluconic acid chelate for chloro-organic destruction by modified Fenton reaction",
abstract = "Various remediation technologies are being developed for the destruction of groundwater pollutants like 2,4,6-trichlorophenol (TCP) and polychlorinated biphenyls (PCBs). This paper is focused on the oxidative dechlorination of TCP by chelate-based Fenton reaction near neutral pH using enzymatically generated hydrogen peroxide and chelate gluconic acid. For the application of Fenton reaction in in-situ remediation, its successful operation near neutral pH is essential. Hence, Fenton reaction is carried out in the presence of chelates that sequester ferrous and ferric ions, making its operation near neutral pH possible. The on-site simultaneous production of hydrogen peroxide and a non-toxic chelate for in-situ remediation of toxic organics by Fenton reaction is highly desirable. For this purpose, well-known enzyme glucose oxidase (GOx), and inexpensive reactants such as glucose and air were used. The study involved developing the correct protocols for the simultaneous enzymatic generation of hydrogen peroxide and chelate gluconic acid for the dechlorination of chlorinated organics by Fenton reaction. The oxidation of glucose was carried out using the enzyme in free and immobilized forms. The rate of production of hydrogen peroxide was determined for the homogeneous and heterogeneous systems, and was used to estimate the time required for complete consumption of glucose during the process, thus avoiding any traces of glucose in the Fenton reaction. The Fenton reaction was carried out at varying ratios of gluconic acid:Fe and under different pH conditions, and their effect on the decomposition of TCP and H 2O 2 was studied. The reaction rate was modeled in terms of the oxidation of parent compound TCP and decomposition of H 2O 2.",
keywords = "Chelate, Enzyme glucose oxidase, Fenton reaction, Gluconic acid, Hydrogen peroxide, Immobilization, pH",
author = "Deepak Ahuja and Bachas, {Leonidas G} and D. Bhattacharyya",
year = "2005",
language = "English (US)",
pages = "8225--8230",
booktitle = "AIChE Annual Meeting, Conference Proceedings",

}

TY - GEN

T1 - Enzymatic generation of hydrogen peroxide and gluconic acid chelate for chloro-organic destruction by modified Fenton reaction

AU - Ahuja, Deepak

AU - Bachas, Leonidas G

AU - Bhattacharyya, D.

PY - 2005

Y1 - 2005

N2 - Various remediation technologies are being developed for the destruction of groundwater pollutants like 2,4,6-trichlorophenol (TCP) and polychlorinated biphenyls (PCBs). This paper is focused on the oxidative dechlorination of TCP by chelate-based Fenton reaction near neutral pH using enzymatically generated hydrogen peroxide and chelate gluconic acid. For the application of Fenton reaction in in-situ remediation, its successful operation near neutral pH is essential. Hence, Fenton reaction is carried out in the presence of chelates that sequester ferrous and ferric ions, making its operation near neutral pH possible. The on-site simultaneous production of hydrogen peroxide and a non-toxic chelate for in-situ remediation of toxic organics by Fenton reaction is highly desirable. For this purpose, well-known enzyme glucose oxidase (GOx), and inexpensive reactants such as glucose and air were used. The study involved developing the correct protocols for the simultaneous enzymatic generation of hydrogen peroxide and chelate gluconic acid for the dechlorination of chlorinated organics by Fenton reaction. The oxidation of glucose was carried out using the enzyme in free and immobilized forms. The rate of production of hydrogen peroxide was determined for the homogeneous and heterogeneous systems, and was used to estimate the time required for complete consumption of glucose during the process, thus avoiding any traces of glucose in the Fenton reaction. The Fenton reaction was carried out at varying ratios of gluconic acid:Fe and under different pH conditions, and their effect on the decomposition of TCP and H 2O 2 was studied. The reaction rate was modeled in terms of the oxidation of parent compound TCP and decomposition of H 2O 2.

AB - Various remediation technologies are being developed for the destruction of groundwater pollutants like 2,4,6-trichlorophenol (TCP) and polychlorinated biphenyls (PCBs). This paper is focused on the oxidative dechlorination of TCP by chelate-based Fenton reaction near neutral pH using enzymatically generated hydrogen peroxide and chelate gluconic acid. For the application of Fenton reaction in in-situ remediation, its successful operation near neutral pH is essential. Hence, Fenton reaction is carried out in the presence of chelates that sequester ferrous and ferric ions, making its operation near neutral pH possible. The on-site simultaneous production of hydrogen peroxide and a non-toxic chelate for in-situ remediation of toxic organics by Fenton reaction is highly desirable. For this purpose, well-known enzyme glucose oxidase (GOx), and inexpensive reactants such as glucose and air were used. The study involved developing the correct protocols for the simultaneous enzymatic generation of hydrogen peroxide and chelate gluconic acid for the dechlorination of chlorinated organics by Fenton reaction. The oxidation of glucose was carried out using the enzyme in free and immobilized forms. The rate of production of hydrogen peroxide was determined for the homogeneous and heterogeneous systems, and was used to estimate the time required for complete consumption of glucose during the process, thus avoiding any traces of glucose in the Fenton reaction. The Fenton reaction was carried out at varying ratios of gluconic acid:Fe and under different pH conditions, and their effect on the decomposition of TCP and H 2O 2 was studied. The reaction rate was modeled in terms of the oxidation of parent compound TCP and decomposition of H 2O 2.

KW - Chelate

KW - Enzyme glucose oxidase

KW - Fenton reaction

KW - Gluconic acid

KW - Hydrogen peroxide

KW - Immobilization

KW - pH

UR - http://www.scopus.com/inward/record.url?scp=33645639351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645639351&partnerID=8YFLogxK

M3 - Conference contribution

SP - 8225

EP - 8230

BT - AIChE Annual Meeting, Conference Proceedings

ER -