Enzymatic Degradation of Hydroxamate in Rumen Microbes

H. P.S. Makkar, O. P. Sharma, Rajinder Dawra

Research output: Contribution to journalArticle

Abstract

The enzyme responsible for degradation of acetohydroxamic acid was intracellular. Bacterial and not the protozoal fraction contributed the enzyme activity in the rumen. The enzyme was cell bound and required cofactors in the soluble portion of the cell for activity. Optimum pH and temperature were 7.0 and 39°C. The enzyme was heat stable. No loss of activity was observed after heating at 70 and 100°C for 10 and 5 min, respectively. On storing enzyme at 25°C, loss of activity was 50% on 2nd day, and on 3rd day there was complete loss of activity. There was no loss of activity on storing at 8°C for 3 days. The enzyme lost activity on freezing and thawing. The chelating agents and sulfhydryl agents were inhibitory in nature. Lead2+, mercury2+, silver2+, cadmium2+, zinc2+, cobalt2+, and nickel2+ inhibited the enzyme. Manganese2+, magnesium 2+, and calcium2+ did not have any effect as high as 100 mM. Among manganese2+, magnesium2+, and calcium2+, only calcium2+ was able to restore activity of ethylenediaminetetraacetate-treated enzyme, suggesting calcium2+ was the cofactor. Nitrophenol, (o and p), dinitrophenol (2, 4 and 2, 6), and sodium cyanide were strong inhibitors of the enzyme, whereas glucose and hydroxylamine did not have any effect. The rate of hydrolysis of different hydroxamates was in order: nicotinohydroxamic acid > phenylacetohydroxamic acid > caprylohydroxamic acid > benzohydroxamic acid > acetohydroxamic acid.

Original languageEnglish (US)
Pages (from-to)2337-2342
Number of pages6
JournalJournal of Dairy Science
Volume66
Issue number11
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

Rumen
rumen
microorganisms
degradation
Enzymes
enzymes
acids
nitrophenols
2,4-dinitrophenol
heat
Sodium Cyanide
2,4-Dinitrophenol
enzyme inhibitors
cyanides
Hydroxylamine
chelating agents
thawing
Enzyme Inhibitors
Chelating Agents
magnesium

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Enzymatic Degradation of Hydroxamate in Rumen Microbes. / Makkar, H. P.S.; Sharma, O. P.; Dawra, Rajinder.

In: Journal of Dairy Science, Vol. 66, No. 11, 01.01.1983, p. 2337-2342.

Research output: Contribution to journalArticle

Makkar, H. P.S. ; Sharma, O. P. ; Dawra, Rajinder. / Enzymatic Degradation of Hydroxamate in Rumen Microbes. In: Journal of Dairy Science. 1983 ; Vol. 66, No. 11. pp. 2337-2342.
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