Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells

Mechanism for persistence in AIDS

Mario Stevenson, Craig Meier, Angela M. Mann, Nora Chapman, Andrzej Wasiak

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Masking of host cell receptors following retroviral infection is the basis for the phenomenon of virus interference. Amphotropic retrovirus vectors were used to express the HIV envelope glycoprotein in a human CD4+ cell line. Envelope expression is accompanied by a reduction in the level of surface CD4 receptor molecules and correlates with the presence of intracellular envelope-CD4 receptor complexes. Cells expressing the HIV envelope acquire a cytolysis-resistant phenotype such that infection with HIV leads to a noncytopathic persistent virus infection. Furthermore, phorbol ester-mediated stimulation of viral replication in persistently infected cells results in renewed cytolytic effects which, due to the absence of CD4 in the cell population, are absolutely independent of syncytium formation. This study elucidates the mechanism by which viral persistence is initiated and maintained in the course of AIDS.

Original languageEnglish
Pages (from-to)483-496
Number of pages14
JournalCell
Volume53
Issue number3
DOIs
StatePublished - May 6 1988
Externally publishedYes

Fingerprint

CD4 Antigens
Glycoproteins
Acquired Immunodeficiency Syndrome
Cells
HIV
Viruses
Phorbol Esters
Virus Diseases
Giant Cells
Retroviridae
HIV Infections
Molecules
Phenotype
Cell Line
Infection
Population

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells : Mechanism for persistence in AIDS. / Stevenson, Mario; Meier, Craig; Mann, Angela M.; Chapman, Nora; Wasiak, Andrzej.

In: Cell, Vol. 53, No. 3, 06.05.1988, p. 483-496.

Research output: Contribution to journalArticle

Stevenson, Mario ; Meier, Craig ; Mann, Angela M. ; Chapman, Nora ; Wasiak, Andrzej. / Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells : Mechanism for persistence in AIDS. In: Cell. 1988 ; Vol. 53, No. 3. pp. 483-496.
@article{a5c520a0b1ff495db380745cf48012ac,
title = "Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells: Mechanism for persistence in AIDS",
abstract = "Masking of host cell receptors following retroviral infection is the basis for the phenomenon of virus interference. Amphotropic retrovirus vectors were used to express the HIV envelope glycoprotein in a human CD4+ cell line. Envelope expression is accompanied by a reduction in the level of surface CD4 receptor molecules and correlates with the presence of intracellular envelope-CD4 receptor complexes. Cells expressing the HIV envelope acquire a cytolysis-resistant phenotype such that infection with HIV leads to a noncytopathic persistent virus infection. Furthermore, phorbol ester-mediated stimulation of viral replication in persistently infected cells results in renewed cytolytic effects which, due to the absence of CD4 in the cell population, are absolutely independent of syncytium formation. This study elucidates the mechanism by which viral persistence is initiated and maintained in the course of AIDS.",
author = "Mario Stevenson and Craig Meier and Mann, {Angela M.} and Nora Chapman and Andrzej Wasiak",
year = "1988",
month = "5",
day = "6",
doi = "10.1016/0092-8674(88)90168-7",
language = "English",
volume = "53",
pages = "483--496",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells

T2 - Mechanism for persistence in AIDS

AU - Stevenson, Mario

AU - Meier, Craig

AU - Mann, Angela M.

AU - Chapman, Nora

AU - Wasiak, Andrzej

PY - 1988/5/6

Y1 - 1988/5/6

N2 - Masking of host cell receptors following retroviral infection is the basis for the phenomenon of virus interference. Amphotropic retrovirus vectors were used to express the HIV envelope glycoprotein in a human CD4+ cell line. Envelope expression is accompanied by a reduction in the level of surface CD4 receptor molecules and correlates with the presence of intracellular envelope-CD4 receptor complexes. Cells expressing the HIV envelope acquire a cytolysis-resistant phenotype such that infection with HIV leads to a noncytopathic persistent virus infection. Furthermore, phorbol ester-mediated stimulation of viral replication in persistently infected cells results in renewed cytolytic effects which, due to the absence of CD4 in the cell population, are absolutely independent of syncytium formation. This study elucidates the mechanism by which viral persistence is initiated and maintained in the course of AIDS.

AB - Masking of host cell receptors following retroviral infection is the basis for the phenomenon of virus interference. Amphotropic retrovirus vectors were used to express the HIV envelope glycoprotein in a human CD4+ cell line. Envelope expression is accompanied by a reduction in the level of surface CD4 receptor molecules and correlates with the presence of intracellular envelope-CD4 receptor complexes. Cells expressing the HIV envelope acquire a cytolysis-resistant phenotype such that infection with HIV leads to a noncytopathic persistent virus infection. Furthermore, phorbol ester-mediated stimulation of viral replication in persistently infected cells results in renewed cytolytic effects which, due to the absence of CD4 in the cell population, are absolutely independent of syncytium formation. This study elucidates the mechanism by which viral persistence is initiated and maintained in the course of AIDS.

UR - http://www.scopus.com/inward/record.url?scp=0023933994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023933994&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(88)90168-7

DO - 10.1016/0092-8674(88)90168-7

M3 - Article

VL - 53

SP - 483

EP - 496

JO - Cell

JF - Cell

SN - 0092-8674

IS - 3

ER -