Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus

Mauro Pistello, Francesca Bonci, Elisa Zabogli, Francesca Conti, Giulia Freer, Fabrizio Maggi, Mario Stevenson, Mauro Bendinelli

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The envelope (Env) glycoproteins of HIV and other lentiviruses possess neutralization and other protective epitopes, yet all attempts to induce protective immunity using Env as the only immunogen have either failed or afforded minimal levels of protection. In a novel prime-boost approach, specific-pathogen-free cats were primed with a plasmid expressing Env of feline immunodeficiency virus (FIV) and feline granulocyte-macrophage colony-stimulating factor and then boosted with their own T lymphocytes transduced ex vivo to produce the same Env and interleukin 15 (3 X 106 to 10 X 106 viable cells/cat). After the boost, the vaccinees developed elevated immune responses, including virus-neutralizing antibodies (NA). Challenge with an ex vivo preparation of FIV readily infected all eight control cats (four mock vaccinated and four naive) and produced a marked decline in the proportion of peripheral CD4 T cells. In contrast, five of seven vaccinees showed little or no traces of infection, and the remaining two had reduced viral loads and underwent no changes in proportions of CD4 T cells. Interestingly, the viral loads of the vaccinees were inversely correlated to the titers of NA. The findings support the concept that Env is a valuable immunogen but needs to be administered in a way that permits the expression of its full protective potential.

Original languageEnglish
Pages (from-to)3845-3856
Number of pages12
JournalJournal of Virology
Volume84
Issue number8
DOIs
StatePublished - Apr 1 2010
Externally publishedYes

Fingerprint

Feline Immunodeficiency Virus
Feline immunodeficiency virus
Neutralizing Antibodies
neutralizing antibodies
Cats
T-lymphocytes
cats
Viral Load
T-Lymphocytes
viral load
Specific Pathogen-Free Organisms
Interleukin-15
Lentivirus
Interleukin-3
Felidae
Granulocyte-Macrophage Colony-Stimulating Factor
antigens
granulocyte-macrophage colony-stimulating factor
Epitopes
Immunity

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus. / Pistello, Mauro; Bonci, Francesca; Zabogli, Elisa; Conti, Francesca; Freer, Giulia; Maggi, Fabrizio; Stevenson, Mario; Bendinelli, Mauro.

In: Journal of Virology, Vol. 84, No. 8, 01.04.2010, p. 3845-3856.

Research output: Contribution to journalArticle

Pistello, Mauro ; Bonci, Francesca ; Zabogli, Elisa ; Conti, Francesca ; Freer, Giulia ; Maggi, Fabrizio ; Stevenson, Mario ; Bendinelli, Mauro. / Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus. In: Journal of Virology. 2010 ; Vol. 84, No. 8. pp. 3845-3856.
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