Abstract
Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 135-140 |
| Number of pages | 6 |
| Journal | Micron (1969) |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1983 |
Keywords
- Microprocesses
- morphometry
- T-lymphocyte
ASJC Scopus subject areas
- Cell Biology
- Materials Science(all)
- Instrumentation
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Okagaki, T., Clark, B. A., Twiggs, L. B., & Condie, R. M. (1983). Enumeration of the number of microprocesses of cultured murine T-lymphoma cells: A comparative study of two new morphometric methods. Micron (1969), 14(2), 135-140. https://doi.org/10.1016/0047-7206(83)90015-8