Abstract
Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.
| Original language | English |
|---|---|
| Pages (from-to) | 135-140 |
| Number of pages | 6 |
| Journal | Micron (1969) |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1983 |
| Externally published | Yes |
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Keywords
- Microprocesses
- morphometry
- T-lymphocyte
ASJC Scopus subject areas
- Cell Biology
- Materials Science(all)
- Instrumentation
Cite this
Enumeration of the number of microprocesses of cultured murine T-lymphoma cells : A comparative study of two new morphometric methods. / Okagaki, Takashi; Clark, Barbara A.; Twiggs, Leo B.; Condie, Richard M.
In: Micron (1969), Vol. 14, No. 2, 01.01.1983, p. 135-140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enumeration of the number of microprocesses of cultured murine T-lymphoma cells
T2 - A comparative study of two new morphometric methods
AU - Okagaki, Takashi
AU - Clark, Barbara A.
AU - Twiggs, Leo B.
AU - Condie, Richard M.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.
AB - Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.
KW - Microprocesses
KW - morphometry
KW - T-lymphocyte
UR - http://www.scopus.com/inward/record.url?scp=0020963917&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020963917&partnerID=8YFLogxK
U2 - 10.1016/0047-7206(83)90015-8
DO - 10.1016/0047-7206(83)90015-8
M3 - Article
AN - SCOPUS:0020963917
VL - 14
SP - 135
EP - 140
JO - Micron (1969)
JF - Micron (1969)
SN - 0047-7206
IS - 2
ER -