Enumeration of the number of microprocesses of cultured murine T-lymphoma cells: A comparative study of two new morphometric methods

Takashi Okagaki, Barbara A. Clark, Leo B. Twiggs, Richard M. Condie

Research output: Contribution to journalArticle

Abstract

Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalMicron (1969)
Volume14
Issue number2
DOIs
StatePublished - 1983

Keywords

  • Microprocesses
  • morphometry
  • T-lymphocyte

ASJC Scopus subject areas

  • Cell Biology
  • Materials Science(all)
  • Instrumentation

Fingerprint Dive into the research topics of 'Enumeration of the number of microprocesses of cultured murine T-lymphoma cells: A comparative study of two new morphometric methods'. Together they form a unique fingerprint.

  • Cite this