Enumeration of the number of microprocesses of cultured murine T-lymphoma cells: A comparative study of two new morphometric methods

Takashi Okagaki, Barbara A. Clark, Leo B. Twiggs, Richard M. Condie

Research output: Contribution to journalArticle

Abstract

Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.

Original languageEnglish
Pages (from-to)135-140
Number of pages6
JournalMicron (1969)
Volume14
Issue number2
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

enumeration
T-Cell Lymphoma
Electron microscopes
electron microscopes
Electrons
cells
Scanning
scanning
cultured cells
porosity

Keywords

  • Microprocesses
  • morphometry
  • T-lymphocyte

ASJC Scopus subject areas

  • Cell Biology
  • Materials Science(all)
  • Instrumentation

Cite this

Enumeration of the number of microprocesses of cultured murine T-lymphoma cells : A comparative study of two new morphometric methods. / Okagaki, Takashi; Clark, Barbara A.; Twiggs, Leo B.; Condie, Richard M.

In: Micron (1969), Vol. 14, No. 2, 01.01.1983, p. 135-140.

Research output: Contribution to journalArticle

Okagaki, Takashi ; Clark, Barbara A. ; Twiggs, Leo B. ; Condie, Richard M. / Enumeration of the number of microprocesses of cultured murine T-lymphoma cells : A comparative study of two new morphometric methods. In: Micron (1969). 1983 ; Vol. 14, No. 2. pp. 135-140.
@article{1f2d143c0cb54271a8ad462f401e07b7,
title = "Enumeration of the number of microprocesses of cultured murine T-lymphoma cells: A comparative study of two new morphometric methods",
abstract = "Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.",
keywords = "Microprocesses, morphometry, T-lymphocyte",
author = "Takashi Okagaki and Clark, {Barbara A.} and Twiggs, {Leo B.} and Condie, {Richard M.}",
year = "1983",
month = "1",
day = "1",
doi = "10.1016/0047-7206(83)90015-8",
language = "English",
volume = "14",
pages = "135--140",
journal = "Micron (1969)",
issn = "0047-7206",
publisher = "Pergamon Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Enumeration of the number of microprocesses of cultured murine T-lymphoma cells

T2 - A comparative study of two new morphometric methods

AU - Okagaki, Takashi

AU - Clark, Barbara A.

AU - Twiggs, Leo B.

AU - Condie, Richard M.

PY - 1983/1/1

Y1 - 1983/1/1

N2 - Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.

AB - Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.

KW - Microprocesses

KW - morphometry

KW - T-lymphocyte

UR - http://www.scopus.com/inward/record.url?scp=0020963917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020963917&partnerID=8YFLogxK

U2 - 10.1016/0047-7206(83)90015-8

DO - 10.1016/0047-7206(83)90015-8

M3 - Article

AN - SCOPUS:0020963917

VL - 14

SP - 135

EP - 140

JO - Micron (1969)

JF - Micron (1969)

SN - 0047-7206

IS - 2

ER -