Abstract
Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured in vitro were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.
Original language | English (US) |
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Pages (from-to) | 135-140 |
Number of pages | 6 |
Journal | Micron (1969) |
Volume | 14 |
Issue number | 2 |
DOIs | |
State | Published - 1983 |
Externally published | Yes |
Keywords
- Microprocesses
- morphometry
- T-lymphocyte
ASJC Scopus subject areas
- Cell Biology
- Materials Science(all)
- Instrumentation