Enterobacter sakazakii enhances epithelial cell injury by inducing apoptosis in a rat model of necrotizing enterocolitis

Catherine J. Hunter, Vijay K. Singamsetty, Nikunj K. Chokshi, Patricia Boyle, Victoria Camerini, Anatoly V. Grishin, Jeffrey S. Upperman, Henri Ford, Nemani V. Prasadarao

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Necrotizing enterocolitis (NEC) is an inflammatory intestinal disorder that affects 2%-5% of all premature infants. Enterobacter sakazakii, a common contaminant of milk-based powdered infant formula, has been implicated as a causative agent of sepsis, meningitis, and NEC in newborn infants, with high mortality rates. However, the role played by E. sakazakii in the pathogenesis of NEC is, to date, not known. Here, we demonstrate for the first time that E. sakazakii can induce clinical and histological NEC in newborn rats. E. sakazakii was found to bind to enterocytes in rat pups at the tips of villi and to intestinal epithelial cells (IEC-6) in culture, with no significant invasion. Exposure to E. sakazakii induced apoptosis and increased the production of interleukin-6 in IEC-6 cells and in the animal model. These data suggest that E. sakazakii could be a potential pathogen that induces NEC and triggers intestinal disease by modulating enterocyte intracellular signaling pathways.

Original languageEnglish (US)
Pages (from-to)586-593
Number of pages8
JournalJournal of Infectious Diseases
Volume198
Issue number4
DOIs
StatePublished - Aug 15 2008
Externally publishedYes

Fingerprint

Cronobacter sakazakii
Necrotizing Enterocolitis
Epithelial Cells
Apoptosis
Wounds and Injuries
Enterocytes
Intestinal Diseases
Infant Formula
Meningitis
Premature Infants
Interleukin-6
Sepsis
Milk
Animal Models
Newborn Infant
Mortality

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Hunter, C. J., Singamsetty, V. K., Chokshi, N. K., Boyle, P., Camerini, V., Grishin, A. V., ... Prasadarao, N. V. (2008). Enterobacter sakazakii enhances epithelial cell injury by inducing apoptosis in a rat model of necrotizing enterocolitis. Journal of Infectious Diseases, 198(4), 586-593. https://doi.org/10.1086/590186

Enterobacter sakazakii enhances epithelial cell injury by inducing apoptosis in a rat model of necrotizing enterocolitis. / Hunter, Catherine J.; Singamsetty, Vijay K.; Chokshi, Nikunj K.; Boyle, Patricia; Camerini, Victoria; Grishin, Anatoly V.; Upperman, Jeffrey S.; Ford, Henri; Prasadarao, Nemani V.

In: Journal of Infectious Diseases, Vol. 198, No. 4, 15.08.2008, p. 586-593.

Research output: Contribution to journalArticle

Hunter, CJ, Singamsetty, VK, Chokshi, NK, Boyle, P, Camerini, V, Grishin, AV, Upperman, JS, Ford, H & Prasadarao, NV 2008, 'Enterobacter sakazakii enhances epithelial cell injury by inducing apoptosis in a rat model of necrotizing enterocolitis', Journal of Infectious Diseases, vol. 198, no. 4, pp. 586-593. https://doi.org/10.1086/590186
Hunter, Catherine J. ; Singamsetty, Vijay K. ; Chokshi, Nikunj K. ; Boyle, Patricia ; Camerini, Victoria ; Grishin, Anatoly V. ; Upperman, Jeffrey S. ; Ford, Henri ; Prasadarao, Nemani V. / Enterobacter sakazakii enhances epithelial cell injury by inducing apoptosis in a rat model of necrotizing enterocolitis. In: Journal of Infectious Diseases. 2008 ; Vol. 198, No. 4. pp. 586-593.
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