eNOS-dependent vascular interaction between nitric oxide and calcitonin gene-related peptide in mice

Gender selectivity and effects on blood aggregation

Woo In Lee, Ye Xu, Sun Mi Fung, Ho Leung Fung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The present study was performed to explore a possible vascular interplay between nitric oxide (NO) and calcitonin gene-related peptide (CGRP). We examined factors affecting CGRP release by the NO donor, nitroglycerin (NTG) and the potential involvement of endothelial NO synthase (eNOS) using eNOS knockout (-/-) vs. wild-type (+/+) mice. In the female eNOS (+/+) mice, but not in males, in vitro NTG (0.73 mM) induced significant increases in the release of CGRP-like immunoreactivity (CGRP-LI) from the aorta and the heart but not from the small intestine. In eNOS (-/-) mice, NTG incubation did not induce any CGRP-LI changes in either gender. These results suggest that NTG-induced CGRP release is eNOS-dependent and tissue- and gender-selective. The functional implication of this NO-CGRP interaction was further examined by testing the anti-aggregatory action of acetylcholine (Ach). Ach-induced platelet inhibition was significantly enhanced by the addition of aorta segments of either gender. However, the female aorta segments exhibited a greater platelet inhibitory effect, which could be reversed by the blockade of either CGRP or eNOS. Our study revealed a novel eNOS-dependent interaction between NO and CGRP, and the possible participation of regulatory peptides in affecting platelet function and possibly cardiovascular protection in females.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalRegulatory Peptides
Volume110
Issue number2
DOIs
StatePublished - Jan 31 2003
Externally publishedYes

Fingerprint

Calcitonin Gene-Related Peptide
Nitric Oxide Synthase
Blood Vessels
Nitric Oxide
Blood
Agglomeration
Nitroglycerin
Platelets
Aorta
Blood Platelets
Acetylcholine
Nitric Oxide Donors
Nitric Oxide Synthase Type III
Small Intestine
Tissue
Peptides
Testing

Keywords

  • Acetylcholine
  • Knockout
  • Nitroglycerin
  • Platelet inhibition
  • Vasculature

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

eNOS-dependent vascular interaction between nitric oxide and calcitonin gene-related peptide in mice : Gender selectivity and effects on blood aggregation. / Lee, Woo In; Xu, Ye; Fung, Sun Mi; Fung, Ho Leung.

In: Regulatory Peptides, Vol. 110, No. 2, 31.01.2003, p. 115-122.

Research output: Contribution to journalArticle

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