eNOS-dependent vascular interaction between nitric oxide and calcitonin gene-related peptide in mice: Gender selectivity and effects on blood aggregation

Woo In Lee, Ye Xu, Sun Mi Fung, Ho Leung Fung

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The present study was performed to explore a possible vascular interplay between nitric oxide (NO) and calcitonin gene-related peptide (CGRP). We examined factors affecting CGRP release by the NO donor, nitroglycerin (NTG) and the potential involvement of endothelial NO synthase (eNOS) using eNOS knockout (-/-) vs. wild-type (+/+) mice. In the female eNOS (+/+) mice, but not in males, in vitro NTG (0.73 mM) induced significant increases in the release of CGRP-like immunoreactivity (CGRP-LI) from the aorta and the heart but not from the small intestine. In eNOS (-/-) mice, NTG incubation did not induce any CGRP-LI changes in either gender. These results suggest that NTG-induced CGRP release is eNOS-dependent and tissue- and gender-selective. The functional implication of this NO-CGRP interaction was further examined by testing the anti-aggregatory action of acetylcholine (Ach). Ach-induced platelet inhibition was significantly enhanced by the addition of aorta segments of either gender. However, the female aorta segments exhibited a greater platelet inhibitory effect, which could be reversed by the blockade of either CGRP or eNOS. Our study revealed a novel eNOS-dependent interaction between NO and CGRP, and the possible participation of regulatory peptides in affecting platelet function and possibly cardiovascular protection in females.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalRegulatory Peptides
Volume110
Issue number2
DOIs
StatePublished - Jan 31 2003
Externally publishedYes

Keywords

  • Acetylcholine
  • Knockout
  • Nitroglycerin
  • Platelet inhibition
  • Vasculature

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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