Abstract
The present study was performed to explore a possible vascular interplay between nitric oxide (NO) and calcitonin gene-related peptide (CGRP). We examined factors affecting CGRP release by the NO donor, nitroglycerin (NTG) and the potential involvement of endothelial NO synthase (eNOS) using eNOS knockout (-/-) vs. wild-type (+/+) mice. In the female eNOS (+/+) mice, but not in males, in vitro NTG (0.73 mM) induced significant increases in the release of CGRP-like immunoreactivity (CGRP-LI) from the aorta and the heart but not from the small intestine. In eNOS (-/-) mice, NTG incubation did not induce any CGRP-LI changes in either gender. These results suggest that NTG-induced CGRP release is eNOS-dependent and tissue- and gender-selective. The functional implication of this NO-CGRP interaction was further examined by testing the anti-aggregatory action of acetylcholine (Ach). Ach-induced platelet inhibition was significantly enhanced by the addition of aorta segments of either gender. However, the female aorta segments exhibited a greater platelet inhibitory effect, which could be reversed by the blockade of either CGRP or eNOS. Our study revealed a novel eNOS-dependent interaction between NO and CGRP, and the possible participation of regulatory peptides in affecting platelet function and possibly cardiovascular protection in females.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 115-122 |
| Number of pages | 8 |
| Journal | Regulatory Peptides |
| Volume | 110 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 31 2003 |
| Externally published | Yes |
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Keywords
- Acetylcholine
- Knockout
- Nitroglycerin
- Platelet inhibition
- Vasculature
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Neuroscience(all)
Cite this
eNOS-dependent vascular interaction between nitric oxide and calcitonin gene-related peptide in mice : Gender selectivity and effects on blood aggregation. / Lee, Woo In; Xu, Ye; Fung, Sun Mi; Fung, Ho Leung.
In: Regulatory Peptides, Vol. 110, No. 2, 31.01.2003, p. 115-122.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - eNOS-dependent vascular interaction between nitric oxide and calcitonin gene-related peptide in mice
T2 - Gender selectivity and effects on blood aggregation
AU - Lee, Woo In
AU - Xu, Ye
AU - Fung, Sun Mi
AU - Fung, Ho Leung
PY - 2003/1/31
Y1 - 2003/1/31
N2 - The present study was performed to explore a possible vascular interplay between nitric oxide (NO) and calcitonin gene-related peptide (CGRP). We examined factors affecting CGRP release by the NO donor, nitroglycerin (NTG) and the potential involvement of endothelial NO synthase (eNOS) using eNOS knockout (-/-) vs. wild-type (+/+) mice. In the female eNOS (+/+) mice, but not in males, in vitro NTG (0.73 mM) induced significant increases in the release of CGRP-like immunoreactivity (CGRP-LI) from the aorta and the heart but not from the small intestine. In eNOS (-/-) mice, NTG incubation did not induce any CGRP-LI changes in either gender. These results suggest that NTG-induced CGRP release is eNOS-dependent and tissue- and gender-selective. The functional implication of this NO-CGRP interaction was further examined by testing the anti-aggregatory action of acetylcholine (Ach). Ach-induced platelet inhibition was significantly enhanced by the addition of aorta segments of either gender. However, the female aorta segments exhibited a greater platelet inhibitory effect, which could be reversed by the blockade of either CGRP or eNOS. Our study revealed a novel eNOS-dependent interaction between NO and CGRP, and the possible participation of regulatory peptides in affecting platelet function and possibly cardiovascular protection in females.
AB - The present study was performed to explore a possible vascular interplay between nitric oxide (NO) and calcitonin gene-related peptide (CGRP). We examined factors affecting CGRP release by the NO donor, nitroglycerin (NTG) and the potential involvement of endothelial NO synthase (eNOS) using eNOS knockout (-/-) vs. wild-type (+/+) mice. In the female eNOS (+/+) mice, but not in males, in vitro NTG (0.73 mM) induced significant increases in the release of CGRP-like immunoreactivity (CGRP-LI) from the aorta and the heart but not from the small intestine. In eNOS (-/-) mice, NTG incubation did not induce any CGRP-LI changes in either gender. These results suggest that NTG-induced CGRP release is eNOS-dependent and tissue- and gender-selective. The functional implication of this NO-CGRP interaction was further examined by testing the anti-aggregatory action of acetylcholine (Ach). Ach-induced platelet inhibition was significantly enhanced by the addition of aorta segments of either gender. However, the female aorta segments exhibited a greater platelet inhibitory effect, which could be reversed by the blockade of either CGRP or eNOS. Our study revealed a novel eNOS-dependent interaction between NO and CGRP, and the possible participation of regulatory peptides in affecting platelet function and possibly cardiovascular protection in females.
KW - Acetylcholine
KW - Knockout
KW - Nitroglycerin
KW - Platelet inhibition
KW - Vasculature
UR - http://www.scopus.com/inward/record.url?scp=0037473966&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037473966&partnerID=8YFLogxK
U2 - 10.1016/S0167-0115(02)00210-0
DO - 10.1016/S0167-0115(02)00210-0
M3 - Article
C2 - 12527144
AN - SCOPUS:0037473966
VL - 110
SP - 115
EP - 122
JO - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 2
ER -