Enlargement of Lyt-2-positive T cells is associated with functional impairment and autoimmune hemolytic anemia in new Zealand black mice

K. L. McCoy, P. J. Baker, Thomas Malek, T. M. Chused

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We investigated the relationship between the increased cell diameter of Lyt-2+ T cells and the development of autoimmune disease in aging NZB and NZB x NZW F1 hybrid (BW) mice. Individual animals were analyzed for Lyt-2+ T cell size (by narrow-angle forward light scatter), anti-erythrocyte autoantibodies, anemia, proteinuria, and splenomegaly. The peak light scatter of the Lyt-2+ T cells correlated with the level of anti-erythrocyte autoantibodies and severity of hemolytic anemia, but not with proteinuria or splenomegaly. The cell size of this T cell subset did not increase in old BW or in NZB mice homozygous for the xid gene (NZB.xid). The in vivo administration of bacterial lipopolysaccharide to young NZB mice did not stimulate the enlargement of Lyt-2+ T cells. Lyt-2+ cells from old NZB mice could be stimulated by concanavalin A (Con A) to express interleukin 2 (IL 2) receptors and to synthesize DNA in vitro. However, in vivo administration of Con A to old NZB mice did not induce the expression of IL 2 receptors on Lyt-2+ T cells. Further, in vivo T suppressor function was impaired in old NZB mice with enlarged Lyt-2+ T cells. Thus, the enlargement of Lyt-2+ T cells in old NZB mice appears related to impaired T cell function in vivo and is associated with the development of anti-erythrocyte autoantibodies and autoimmune hemolytic anemia.

Original languageEnglish
Pages (from-to)2432-2437
Number of pages6
JournalJournal of Immunology
Volume135
Issue number4
StatePublished - Dec 1 1985
Externally publishedYes

Fingerprint

Autoimmune Hemolytic Anemia
Inbred NZB Mouse
New Zealand
T-Lymphocytes
Autoantibodies
Erythrocytes
Interleukin-2 Receptors
Splenomegaly
Concanavalin A
Cell Size
Proteinuria
Light
Hemolytic Anemia
T-Lymphocyte Subsets
Autoimmune Diseases
Lipopolysaccharides
Anemia
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Enlargement of Lyt-2-positive T cells is associated with functional impairment and autoimmune hemolytic anemia in new Zealand black mice. / McCoy, K. L.; Baker, P. J.; Malek, Thomas; Chused, T. M.

In: Journal of Immunology, Vol. 135, No. 4, 01.12.1985, p. 2432-2437.

Research output: Contribution to journalArticle

@article{95d38a764a23439f89c5618be020a738,
title = "Enlargement of Lyt-2-positive T cells is associated with functional impairment and autoimmune hemolytic anemia in new Zealand black mice",
abstract = "We investigated the relationship between the increased cell diameter of Lyt-2+ T cells and the development of autoimmune disease in aging NZB and NZB x NZW F1 hybrid (BW) mice. Individual animals were analyzed for Lyt-2+ T cell size (by narrow-angle forward light scatter), anti-erythrocyte autoantibodies, anemia, proteinuria, and splenomegaly. The peak light scatter of the Lyt-2+ T cells correlated with the level of anti-erythrocyte autoantibodies and severity of hemolytic anemia, but not with proteinuria or splenomegaly. The cell size of this T cell subset did not increase in old BW or in NZB mice homozygous for the xid gene (NZB.xid). The in vivo administration of bacterial lipopolysaccharide to young NZB mice did not stimulate the enlargement of Lyt-2+ T cells. Lyt-2+ cells from old NZB mice could be stimulated by concanavalin A (Con A) to express interleukin 2 (IL 2) receptors and to synthesize DNA in vitro. However, in vivo administration of Con A to old NZB mice did not induce the expression of IL 2 receptors on Lyt-2+ T cells. Further, in vivo T suppressor function was impaired in old NZB mice with enlarged Lyt-2+ T cells. Thus, the enlargement of Lyt-2+ T cells in old NZB mice appears related to impaired T cell function in vivo and is associated with the development of anti-erythrocyte autoantibodies and autoimmune hemolytic anemia.",
author = "McCoy, {K. L.} and Baker, {P. J.} and Thomas Malek and Chused, {T. M.}",
year = "1985",
month = "12",
day = "1",
language = "English",
volume = "135",
pages = "2432--2437",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Enlargement of Lyt-2-positive T cells is associated with functional impairment and autoimmune hemolytic anemia in new Zealand black mice

AU - McCoy, K. L.

AU - Baker, P. J.

AU - Malek, Thomas

AU - Chused, T. M.

PY - 1985/12/1

Y1 - 1985/12/1

N2 - We investigated the relationship between the increased cell diameter of Lyt-2+ T cells and the development of autoimmune disease in aging NZB and NZB x NZW F1 hybrid (BW) mice. Individual animals were analyzed for Lyt-2+ T cell size (by narrow-angle forward light scatter), anti-erythrocyte autoantibodies, anemia, proteinuria, and splenomegaly. The peak light scatter of the Lyt-2+ T cells correlated with the level of anti-erythrocyte autoantibodies and severity of hemolytic anemia, but not with proteinuria or splenomegaly. The cell size of this T cell subset did not increase in old BW or in NZB mice homozygous for the xid gene (NZB.xid). The in vivo administration of bacterial lipopolysaccharide to young NZB mice did not stimulate the enlargement of Lyt-2+ T cells. Lyt-2+ cells from old NZB mice could be stimulated by concanavalin A (Con A) to express interleukin 2 (IL 2) receptors and to synthesize DNA in vitro. However, in vivo administration of Con A to old NZB mice did not induce the expression of IL 2 receptors on Lyt-2+ T cells. Further, in vivo T suppressor function was impaired in old NZB mice with enlarged Lyt-2+ T cells. Thus, the enlargement of Lyt-2+ T cells in old NZB mice appears related to impaired T cell function in vivo and is associated with the development of anti-erythrocyte autoantibodies and autoimmune hemolytic anemia.

AB - We investigated the relationship between the increased cell diameter of Lyt-2+ T cells and the development of autoimmune disease in aging NZB and NZB x NZW F1 hybrid (BW) mice. Individual animals were analyzed for Lyt-2+ T cell size (by narrow-angle forward light scatter), anti-erythrocyte autoantibodies, anemia, proteinuria, and splenomegaly. The peak light scatter of the Lyt-2+ T cells correlated with the level of anti-erythrocyte autoantibodies and severity of hemolytic anemia, but not with proteinuria or splenomegaly. The cell size of this T cell subset did not increase in old BW or in NZB mice homozygous for the xid gene (NZB.xid). The in vivo administration of bacterial lipopolysaccharide to young NZB mice did not stimulate the enlargement of Lyt-2+ T cells. Lyt-2+ cells from old NZB mice could be stimulated by concanavalin A (Con A) to express interleukin 2 (IL 2) receptors and to synthesize DNA in vitro. However, in vivo administration of Con A to old NZB mice did not induce the expression of IL 2 receptors on Lyt-2+ T cells. Further, in vivo T suppressor function was impaired in old NZB mice with enlarged Lyt-2+ T cells. Thus, the enlargement of Lyt-2+ T cells in old NZB mice appears related to impaired T cell function in vivo and is associated with the development of anti-erythrocyte autoantibodies and autoimmune hemolytic anemia.

UR - http://www.scopus.com/inward/record.url?scp=0022413514&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022413514&partnerID=8YFLogxK

M3 - Article

C2 - 3928748

AN - SCOPUS:0022413514

VL - 135

SP - 2432

EP - 2437

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -