Enkephalin reduces quantal content at the frog neuromuscular junction

John Bixby, N. C. Spitzer

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Opiate peptides, particularly Met-enkephalin, have been shown to block or reduce Ca2+-dependent events in a variety of neurones. Our own observations on the effect of enkephalin on developing Rohon-Beard neurones in Xenopus spinal cord suggested that enkephalin might interact directly with Ca2+ channels in vertebrate neurones. This possibility prompted us to look for an effect of enkephalin on another population of neuronal Ca2+ channels - those in the presynaptic terminals at the frog neuromuscular junction. We were encouraged to try this preparation by an early report that morphine, in rather high concentrations, reduced the amount of acetylcholine (ACh) released from frog muscle by nerve stimulation. Our present results indicate that Met-enkephalin reversibly and specifically reduces the quantal content of transmitter release from nerve terminals in the frog cutaneous pectoris muscle, probably by means of an effect on inward Ca2+ current.

Original languageEnglish
Pages (from-to)431-432
Number of pages2
JournalNature
Volume301
Issue number5899
StatePublished - Jan 1 1983
Externally publishedYes

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Enkephalins
Neuromuscular Junction
Anura
Methionine Enkephalin
Neurons
Muscles
Opioid Peptides
Presynaptic Terminals
Xenopus
Morphine
Acetylcholine
Vertebrates
Spinal Cord
Skin
Population

ASJC Scopus subject areas

  • General

Cite this

Enkephalin reduces quantal content at the frog neuromuscular junction. / Bixby, John; Spitzer, N. C.

In: Nature, Vol. 301, No. 5899, 01.01.1983, p. 431-432.

Research output: Contribution to journalArticle

Bixby, J & Spitzer, NC 1983, 'Enkephalin reduces quantal content at the frog neuromuscular junction', Nature, vol. 301, no. 5899, pp. 431-432.
Bixby, John ; Spitzer, N. C. / Enkephalin reduces quantal content at the frog neuromuscular junction. In: Nature. 1983 ; Vol. 301, No. 5899. pp. 431-432.
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