Enhancing linkage analysis of complex disorders: An evaluation of high-density genotyping

Stephen J. Sawcer, Mel Maranian, Sara Singlehurst, Tai Wai Yeo, Alastair Compston, Mark J. Daly, Philip L. De Jager, Stacey Gabriel, David A. Hafler, Adrian J. Ivinson, Eric S. Lander, John D. Rioux, Emily Walsh, Simon G. Gregory, Silke Schmidt, Margaret A. Pericak-Vance, Lisa Barcellos, Stephen L. Hauser, Jorge R. Oksenberg, Shannon J. KenealyJonathan L. Haines

Research output: Contribution to journalArticlepeer-review

65 Scopus citations


To explore the potential value of recently developed high-density linkage mapping methods in the analysis of complex disease we have regenotyped five nuclear families first studied in the 1996 UK multiple sclerosis linkage genome screen, using Applied Biosystems high-density microsatellite linkage mapping set, the Illumina BeadArray™ linkage mapping panel (version 3) and the Affymetrix GeneChip® Human Mapping 10K array. We found that genotyping success, information extraction and genotyping accuracy were improved with all systems. These improvements were particularly marked with the SNP-based methods (Illumina and Affymetrix), with little difference between these. The extent of additional information extracted is considerable, indicating that reanalysis of existing multiplex families using these newer systems would substantially increase power.

Original languageEnglish (US)
Pages (from-to)1943-1949
Number of pages7
JournalHuman molecular genetics
Issue number17
StatePublished - Sep 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)


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