Enhancing effect of IFN-γ on helper T cell activity and IL 2 production

D. Frasca, L. Adorini, S. Landolfo, G. Doria

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


A single injection of young murine immune interferone (IFN-γ) in young (3 mo) or old (14 to 24 mo) mice 3 days before carrier-priming significantly enhances helper T cell activity of their spleen cells. Maximal enhancement is attained when IFN-γ is injected once immediately before priming or for 4 consecutive days from the time of priming. Helper activity for anti-TNP antibody response was titrated in vitro by adding graded numbers of spleen cells from HRBC-primed mice of a given age to cultures containing a constant number of spleen cells from 3-mo-old normal mice and TNP-HRBC. When T cell-enriched spleen cells from HRBC-primed young or old mice, uninjected or injected with IFN-γ, were separated by nylon wool filtration into passed (Thi) and adherent (Th2) cells, the helper activity of both T cell subpopulations was found to be enhanced by IFN-γ injection. Helper activity of purified Th1 and Th2 cells was also increased by their in vitro preincubation with IFN-γ. Furthermore, interleukin 2 (IL 2) production by mitogen-activated spleen cells from young and old mice is enhanced by addition of IFN-γ to cultures. These data altogether indicate that IFN-γ plays an important role in immunoregulation of helper T cell activity.

Original languageEnglish (US)
Pages (from-to)3907-3911
Number of pages5
JournalJournal of Immunology
Issue number6
StatePublished - Jan 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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