Enhancing effect of IFN-γ on helper T cell activity and IL 2 production

Daniela Frasca, L. Adorini, S. Landolfo, G. Doria

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A single injection of young murine immune interferone (IFN-γ) in young (3 mo) or old (14 to 24 mo) mice 3 days before carrier-priming significantly enhances helper T cell activity of their spleen cells. Maximal enhancement is attained when IFN-γ is injected once immediately before priming or for 4 consecutive days from the time of priming. Helper activity for anti-TNP antibody response was titrated in vitro by adding graded numbers of spleen cells from HRBC-primed mice of a given age to cultures containing a constant number of spleen cells from 3-mo-old normal mice and TNP-HRBC. When T cell-enriched spleen cells from HRBC-primed young or old mice, uninjected or injected with IFN-γ, were separated by nylon wool filtration into passed (Thi) and adherent (Th2) cells, the helper activity of both T cell subpopulations was found to be enhanced by IFN-γ injection. Helper activity of purified Th1 and Th2 cells was also increased by their in vitro preincubation with IFN-γ. Furthermore, interleukin 2 (IL 2) production by mitogen-activated spleen cells from young and old mice is enhanced by addition of IFN-γ to cultures. These data altogether indicate that IFN-γ plays an important role in immunoregulation of helper T cell activity.

Original languageEnglish
Pages (from-to)3907-3911
Number of pages5
JournalJournal of Immunology
Volume134
Issue number6
StatePublished - Jan 1 1985
Externally publishedYes

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Helper-Inducer T-Lymphocytes
Interleukin-2
Spleen
Th2 Cells
Cell Count
T-Lymphocytes
Th1 Cells
Injections
Wool
Nylons
Mitogens
Antibody Formation
Anti-Idiotypic Antibodies
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Frasca, D., Adorini, L., Landolfo, S., & Doria, G. (1985). Enhancing effect of IFN-γ on helper T cell activity and IL 2 production. Journal of Immunology, 134(6), 3907-3911.

Enhancing effect of IFN-γ on helper T cell activity and IL 2 production. / Frasca, Daniela; Adorini, L.; Landolfo, S.; Doria, G.

In: Journal of Immunology, Vol. 134, No. 6, 01.01.1985, p. 3907-3911.

Research output: Contribution to journalArticle

Frasca, D, Adorini, L, Landolfo, S & Doria, G 1985, 'Enhancing effect of IFN-γ on helper T cell activity and IL 2 production', Journal of Immunology, vol. 134, no. 6, pp. 3907-3911.
Frasca, Daniela ; Adorini, L. ; Landolfo, S. ; Doria, G. / Enhancing effect of IFN-γ on helper T cell activity and IL 2 production. In: Journal of Immunology. 1985 ; Vol. 134, No. 6. pp. 3907-3911.
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