Enhancement of UV-induced unscheduled DNA synthesis by hydroxyurea. Demonstration by combined biochemical and autoradiographic techniques

Theodore Lampidis, J. B. Little

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A dual technique for measuring radioactivity incorporated into DNA is presented in which quantitative scintillation counting and autoradiography may be performed on the same preparation of cultured cells. Incubation of UV-irradiated cells with hydroxyurea depressed the incorporation of [3H]thymidine in semi-conservative replication but enhanced incorporation in repair synthesis. A possible mechanism for this increase is discussed. The use of the combination of these simple techniques introduces greater sensitivity and flexibility into the estimation of unscheduled DNA synthesis in cells in various growth states.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalExperimental Cell Research
Volume110
Issue number1
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

Fingerprint

Hydroxyurea
Scintillation Counting
DNA
Autoradiography
Thymidine
Radioactivity
Cultured Cells
Growth

ASJC Scopus subject areas

  • Cell Biology

Cite this

@article{10dd0f17fa1d4f37846b8a82f4b48ebd,
title = "Enhancement of UV-induced unscheduled DNA synthesis by hydroxyurea. Demonstration by combined biochemical and autoradiographic techniques",
abstract = "A dual technique for measuring radioactivity incorporated into DNA is presented in which quantitative scintillation counting and autoradiography may be performed on the same preparation of cultured cells. Incubation of UV-irradiated cells with hydroxyurea depressed the incorporation of [3H]thymidine in semi-conservative replication but enhanced incorporation in repair synthesis. A possible mechanism for this increase is discussed. The use of the combination of these simple techniques introduces greater sensitivity and flexibility into the estimation of unscheduled DNA synthesis in cells in various growth states.",
author = "Theodore Lampidis and Little, {J. B.}",
year = "1977",
month = "1",
day = "1",
doi = "10.1016/0014-4827(77)90267-1",
language = "English",
volume = "110",
pages = "41--46",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Enhancement of UV-induced unscheduled DNA synthesis by hydroxyurea. Demonstration by combined biochemical and autoradiographic techniques

AU - Lampidis, Theodore

AU - Little, J. B.

PY - 1977/1/1

Y1 - 1977/1/1

N2 - A dual technique for measuring radioactivity incorporated into DNA is presented in which quantitative scintillation counting and autoradiography may be performed on the same preparation of cultured cells. Incubation of UV-irradiated cells with hydroxyurea depressed the incorporation of [3H]thymidine in semi-conservative replication but enhanced incorporation in repair synthesis. A possible mechanism for this increase is discussed. The use of the combination of these simple techniques introduces greater sensitivity and flexibility into the estimation of unscheduled DNA synthesis in cells in various growth states.

AB - A dual technique for measuring radioactivity incorporated into DNA is presented in which quantitative scintillation counting and autoradiography may be performed on the same preparation of cultured cells. Incubation of UV-irradiated cells with hydroxyurea depressed the incorporation of [3H]thymidine in semi-conservative replication but enhanced incorporation in repair synthesis. A possible mechanism for this increase is discussed. The use of the combination of these simple techniques introduces greater sensitivity and flexibility into the estimation of unscheduled DNA synthesis in cells in various growth states.

UR - http://www.scopus.com/inward/record.url?scp=0017717401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017717401&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(77)90267-1

DO - 10.1016/0014-4827(77)90267-1

M3 - Article

C2 - 923664

AN - SCOPUS:0017717401

VL - 110

SP - 41

EP - 46

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -