A dual technique for measuring radioactivity incorporated into DNA is presented in which quantitative scintillation counting and autoradiography may be performed on the same preparation of cultured cells. Incubation of UV-irradiated cells with hydroxyurea depressed the incorporation of [3H]thymidine in semi-conservative replication but enhanced incorporation in repair synthesis. A possible mechanism for this increase is discussed. The use of the combination of these simple techniques introduces greater sensitivity and flexibility into the estimation of unscheduled DNA synthesis in cells in various growth states.
ASJC Scopus subject areas
- Cell Biology