Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity

Minas S. Barbarakis, Truis Smith-Palmer, Leonidas G Bachas, Sun Yung Chen, B. Wieb Van Der Meer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.

Original languageEnglish (US)
Pages (from-to)1139-1145
Number of pages7
JournalTalanta
Volume40
Issue number7
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Avidin
Fluorophores
Biotin
Derivatives
Fluorescein
Assays
Fluorescence
Acetic Acid
Calibration
Limit of Detection
Labels

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity. / Barbarakis, Minas S.; Smith-Palmer, Truis; Bachas, Leonidas G; Chen, Sun Yung; Van Der Meer, B. Wieb.

In: Talanta, Vol. 40, No. 7, 1993, p. 1139-1145.

Research output: Contribution to journalArticle

Barbarakis, Minas S. ; Smith-Palmer, Truis ; Bachas, Leonidas G ; Chen, Sun Yung ; Van Der Meer, B. Wieb. / Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity. In: Talanta. 1993 ; Vol. 40, No. 7. pp. 1139-1145.
@article{72c03d55d5164433a2930d8d32c36aba,
title = "Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity",
abstract = "Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.",
author = "Barbarakis, {Minas S.} and Truis Smith-Palmer and Bachas, {Leonidas G} and Chen, {Sun Yung} and {Van Der Meer}, {B. Wieb}",
year = "1993",
doi = "10.1016/0039-9140(93)80177-S",
language = "English (US)",
volume = "40",
pages = "1139--1145",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier",
number = "7",

}

TY - JOUR

T1 - Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity

AU - Barbarakis, Minas S.

AU - Smith-Palmer, Truis

AU - Bachas, Leonidas G

AU - Chen, Sun Yung

AU - Van Der Meer, B. Wieb

PY - 1993

Y1 - 1993

N2 - Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.

AB - Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.

UR - http://www.scopus.com/inward/record.url?scp=0001684747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001684747&partnerID=8YFLogxK

U2 - 10.1016/0039-9140(93)80177-S

DO - 10.1016/0039-9140(93)80177-S

M3 - Article

VL - 40

SP - 1139

EP - 1145

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 7

ER -