TY - JOUR
T1 - Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity
AU - Barbarakis, Minas S.
AU - Smith-Palmer, Truis
AU - Bachas, Leonidas G.
AU - Chen, Sun Yung
AU - Van Der Meer, B. Wieb
N1 - Funding Information:
Acknowledgements-Thriess earchw ass upportedb y grants from theN ationalI nstituteso f Health( GM 40510to LGB), the National ScienceF oundation( EHR-9108764to LGB and BWV), and the Natural Sciencesa nd Engineering ResearchC ouncil (TSP). The authorsk indly acknowledge Dr ParksonC hongf or allowingu s accessto his frequency-domainf luorometer.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1993/7
Y1 - 1993/7
N2 - Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.
AB - Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.
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U2 - 10.1016/0039-9140(93)80177-S
DO - 10.1016/0039-9140(93)80177-S
M3 - Article
AN - SCOPUS:0001684747
VL - 40
SP - 1139
EP - 1145
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 7
ER -