Abstract
Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1139-1145 |
| Number of pages | 7 |
| Journal | Talanta |
| Volume | 40 |
| Issue number | 7 |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
Cite this
Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity. / Barbarakis, Minas S.; Smith-Palmer, Truis; Bachas, Leonidas G; Chen, Sun Yung; Van Der Meer, B. Wieb.
In: Talanta, Vol. 40, No. 7, 1993, p. 1139-1145.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity
AU - Barbarakis, Minas S.
AU - Smith-Palmer, Truis
AU - Bachas, Leonidas G
AU - Chen, Sun Yung
AU - Van Der Meer, B. Wieb
PY - 1993
Y1 - 1993
N2 - Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.
AB - Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.
UR - http://www.scopus.com/inward/record.url?scp=0001684747&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001684747&partnerID=8YFLogxK
U2 - 10.1016/0039-9140(93)80177-S
DO - 10.1016/0039-9140(93)80177-S
M3 - Article
VL - 40
SP - 1139
EP - 1145
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 7
ER -