Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity

Minas S. Barbarakis, Truis Smith-Palmer, Leonidas G. Bachas, Sun Yung Chen, B. Wieb Van Der Meer

Research output: Contribution to journalArticle

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Abstract

Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10-10M.

Original languageEnglish (US)
Pages (from-to)1139-1145
Number of pages7
JournalTalanta
Volume40
Issue number7
DOIs
StatePublished - Jul 1993

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ASJC Scopus subject areas

  • Analytical Chemistry

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