Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity

Minas S. Barbarakis; Truis Smith-Palmer; Leonidas G. Bachas; Sun Yung Chen; B. Wieb Van Der Meer

doi:10.1016/0039-9140(93)80177-S

Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity

Minas S. Barbarakis, Truis Smith-Palmer, Leonidas G. Bachas, Sun Yung Chen, B. Wieb Van Der Meer

Educational and Psychological Studies

Research output: Contribution to journal › Article

16 Scopus citations

Abstract

Fluorescein, Texas Red, Cascade Blue, 7-amino-methylcoumarin-3-acetic acid, and Lucifer Yellow were evaluated as fluorescent labels for homogeneous fluorophore-linked binding assays. Conjugates of avidin with these fluorophores exhibited an enhancement in fluorescence emission in the presence of biotin or biotin derivatives. This property was used in the development of assays for biotin. The biotin-induced fluorescence enhancement of each labeled avidin were compared. Fluorescein led to the most sensitive calibration (dose-response) curve for biotin with a detection limit of 8 × 10^-10M.

Original language	English (US)
Pages (from-to)	1139-1145
Number of pages	7
Journal	Talanta
Volume	40
Issue number	7
DOIs	https://doi.org/10.1016/0039-9140(93)80177-S
State	Published - Jul 1993

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ASJC Scopus subject areas

Analytical Chemistry

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Barbarakis, M. S., Smith-Palmer, T., Bachas, L. G., Chen, S. Y., & Van Der Meer, B. W. (1993). Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity. Talanta, 40(7), 1139-1145. https://doi.org/10.1016/0039-9140(93)80177-S

Enhancement of the emission intensity of fluorophore-labeled avidin by biotin and biotin derivatives. Evaluation of different fluorophores for improved sensitivity. / Barbarakis, Minas S.; Smith-Palmer, Truis; Bachas, Leonidas G.; Chen, Sun Yung; Van Der Meer, B. Wieb.

In: Talanta, Vol. 40, No. 7, 07.1993, p. 1139-1145.

Research output: Contribution to journal › Article

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10.1016/0039-9140(93)80177-S