In the present study, the contribution of IFN-γ to the generation of helper activity in mice was investigated by use of an anti-mouse IFN-γ rat mAb (AN 18.17.24). This mAb was alum precipitated and injected i.p. before or after carrier priming. Results show that spleen cell helper activity is markedly inhibited by anti-IFN-γ mAb injection. This inhibition is time and dose dependent, and counteracted by IFN-γ administration. Thus, the anti-IFN-γ mAb appears to inhibit helper cell activity by neutralization of the IFN-γ required for the antibody response. Moreover, AN 18.17.24 mAb injection results in increased activation of Lyt-2+ T cells which markedly suppress Th activity. These findings altogether indicate that besides the activation of macrophages and Th, IFN-γ seems to exert a negative interference in suppressor T lymphocyte circuits and, as a consequence, to inhibit immunosuppression.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1988|
ASJC Scopus subject areas
- Immunology and Allergy