Enhancement of suppressor T cell activity by injection of anti-IFN-γ monoclonal antibody

Daniela Frasca, L. Adorini, S. Landolfo, G. Doria

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In the present study, the contribution of IFN-γ to the generation of helper activity in mice was investigated by use of an anti-mouse IFN-γ rat mAb (AN 18.17.24). This mAb was alum precipitated and injected i.p. before or after carrier priming. Results show that spleen cell helper activity is markedly inhibited by anti-IFN-γ mAb injection. This inhibition is time and dose dependent, and counteracted by IFN-γ administration. Thus, the anti-IFN-γ mAb appears to inhibit helper cell activity by neutralization of the IFN-γ required for the antibody response. Moreover, AN 18.17.24 mAb injection results in increased activation of Lyt-2+ T cells which markedly suppress Th activity. These findings altogether indicate that besides the activation of macrophages and Th, IFN-γ seems to exert a negative interference in suppressor T lymphocyte circuits and, as a consequence, to inhibit immunosuppression.

Original languageEnglish
Pages (from-to)4103-4107
Number of pages5
JournalJournal of Immunology
Volume140
Issue number12
StatePublished - Jan 1 1988
Externally publishedYes

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Helper-Inducer T-Lymphocytes
Monoclonal Antibodies
T-Lymphocytes
Injections
Macrophage Activation
Immunosuppression
Antibody Formation
Spleen
aluminum sulfate

ASJC Scopus subject areas

  • Immunology

Cite this

Enhancement of suppressor T cell activity by injection of anti-IFN-γ monoclonal antibody. / Frasca, Daniela; Adorini, L.; Landolfo, S.; Doria, G.

In: Journal of Immunology, Vol. 140, No. 12, 01.01.1988, p. 4103-4107.

Research output: Contribution to journalArticle

Frasca, D, Adorini, L, Landolfo, S & Doria, G 1988, 'Enhancement of suppressor T cell activity by injection of anti-IFN-γ monoclonal antibody', Journal of Immunology, vol. 140, no. 12, pp. 4103-4107.
Frasca, Daniela ; Adorini, L. ; Landolfo, S. ; Doria, G. / Enhancement of suppressor T cell activity by injection of anti-IFN-γ monoclonal antibody. In: Journal of Immunology. 1988 ; Vol. 140, No. 12. pp. 4103-4107.
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