Enhancement of antigen presenting ability in the leukemic plasmacytoid dendritic cell line (PMDC05) by lentiviral vector-mediated transduction of CD80 gene

Akie Yamahira, Miwako Narita, Kayoko Ishii, R. M.Chamila Jayathilake, Minami Iwabuchi, Naoya Satoh, Takayoshi Uchiyama, Tomoyo Taniguchi, Shigeo Hashimoto, Noriyuki Kasahara, Emmanuelle Faure, Brooke Bogan, Jun Takizawa, Hirohito Sone, Masuhiro Takahashi

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

PMDC05, a leukemic plasmacytoid dendritic cell (pDC) line which was established in our laboratory, showed a capacity of generating antigen-specific cytotoxic T lymphocytes (CTLs). In order to enhance an antigen presenting ability of PMDC05, PMDC05 was transduced with CD80 gene by lentiviral vector, which was named as PMDC11. PMDC11 displayed a strong antigen presenting ability even without any stimulation, and by culturing with stimulators such as calcium ionophore PMDC11 gained a more potent antigen presenting ability. Our data suggested PMDC11 could be applied as antigen presenting cells more efficiently in adoptive cellular immunotherapy for tumors and severe infections in comparison with PMDC05.

Original languageEnglish (US)
Pages (from-to)1541-1546
Number of pages6
JournalLeukemia Research
Volume36
Issue number12
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

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Keywords

  • Adoptive cellular immunotherapy
  • Antigen presenting ability
  • CD80 gene transduction
  • Lentiviral vector
  • Leukemic plasmacytoid dendritic cell line
  • PMDC05
  • PMDC11

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Yamahira, A., Narita, M., Ishii, K., Jayathilake, R. M. C., Iwabuchi, M., Satoh, N., Uchiyama, T., Taniguchi, T., Hashimoto, S., Kasahara, N., Faure, E., Bogan, B., Takizawa, J., Sone, H., & Takahashi, M. (2012). Enhancement of antigen presenting ability in the leukemic plasmacytoid dendritic cell line (PMDC05) by lentiviral vector-mediated transduction of CD80 gene. Leukemia Research, 36(12), 1541-1546. https://doi.org/10.1016/j.leukres.2012.09.002