Enhanced recovery of cryopreserved islets using SIS

E. J. Woods, C. M. Walsh, R. A. Sidner, M. A J Zieger, S. Mullin, J. R T Lakey, Camillo Ricordi, J. K. Critser

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although cryopreservation of pancreatic islets would add flexibility to transplantation, the recoveries are only 60% to 90% and function is decreased. Islets are multicellular structures ∼50 to 250 μm in diameter organized into a network of cells and vascular channels. Due to this complexity, islets are more susceptible to damage during cryopreservation than an individual cell. This study investigated porcine small intestinal submucosa (SIS) as a matrix to support islets recovery and function post-thaw. Groups of frozen/thawed human islets (150 IE/condition; n = 4 preparations) were cultured for 5 weeks in plates containing noncoated Biopore membrane inserts alone or inserts covered with SIS. Islets were placed directly on the insert post-thaw (SIS1), or cultured overnight in standard plates, washed, and then transferred to the SIS (SIS2). Function was assessed by determining glucose-stimulated release of insulin, which was measured by radioimmunoassay. Analysis of basal insulin secretion showed time and treatment to be significantly different (P = .0043 and P = .0123, respectively) but without an interaction (P > .05). The two SIS treatments were not significantly different (P > .05); however, both SIS1 and SIS2 were significantly different from controls (P = .0108 and P = .0420, respectively). Similar results were obtained for stimulation indices; time and treatment were significantly different (P = .0161 and P = .0264, respectively) but not an interaction (P gt; .05). The two SIS treatments were not significantly different (P = .05); however, both SIS 1 and SIS2 differed from controls (P = .0248 and P = .0407, respectively). The results indicate that SIS enables frozen-thawed islets to exhibit superior post-thaw function compared with a non-SIS-supported condition.

Original languageEnglish
Pages (from-to)1139-1142
Number of pages4
JournalTransplantation Proceedings
Volume36
Issue number4
DOIs
StatePublished - May 1 2004

Fingerprint

Cryopreservation
Insulin
Recovery of Function
Therapeutics
Islets of Langerhans
Radioimmunoassay
Blood Vessels
Swine
Transplantation
Glucose
Membranes

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Woods, E. J., Walsh, C. M., Sidner, R. A., Zieger, M. A. J., Mullin, S., Lakey, J. R. T., ... Critser, J. K. (2004). Enhanced recovery of cryopreserved islets using SIS. Transplantation Proceedings, 36(4), 1139-1142. https://doi.org/10.1016/j.transproceed.2004.05.022

Enhanced recovery of cryopreserved islets using SIS. / Woods, E. J.; Walsh, C. M.; Sidner, R. A.; Zieger, M. A J; Mullin, S.; Lakey, J. R T; Ricordi, Camillo; Critser, J. K.

In: Transplantation Proceedings, Vol. 36, No. 4, 01.05.2004, p. 1139-1142.

Research output: Contribution to journalArticle

Woods, EJ, Walsh, CM, Sidner, RA, Zieger, MAJ, Mullin, S, Lakey, JRT, Ricordi, C & Critser, JK 2004, 'Enhanced recovery of cryopreserved islets using SIS', Transplantation Proceedings, vol. 36, no. 4, pp. 1139-1142. https://doi.org/10.1016/j.transproceed.2004.05.022
Woods EJ, Walsh CM, Sidner RA, Zieger MAJ, Mullin S, Lakey JRT et al. Enhanced recovery of cryopreserved islets using SIS. Transplantation Proceedings. 2004 May 1;36(4):1139-1142. https://doi.org/10.1016/j.transproceed.2004.05.022
Woods, E. J. ; Walsh, C. M. ; Sidner, R. A. ; Zieger, M. A J ; Mullin, S. ; Lakey, J. R T ; Ricordi, Camillo ; Critser, J. K. / Enhanced recovery of cryopreserved islets using SIS. In: Transplantation Proceedings. 2004 ; Vol. 36, No. 4. pp. 1139-1142.
@article{aaf4397a5d964d7e9fe4a4273780ceb9,
title = "Enhanced recovery of cryopreserved islets using SIS",
abstract = "Although cryopreservation of pancreatic islets would add flexibility to transplantation, the recoveries are only 60{\%} to 90{\%} and function is decreased. Islets are multicellular structures ∼50 to 250 μm in diameter organized into a network of cells and vascular channels. Due to this complexity, islets are more susceptible to damage during cryopreservation than an individual cell. This study investigated porcine small intestinal submucosa (SIS) as a matrix to support islets recovery and function post-thaw. Groups of frozen/thawed human islets (150 IE/condition; n = 4 preparations) were cultured for 5 weeks in plates containing noncoated Biopore membrane inserts alone or inserts covered with SIS. Islets were placed directly on the insert post-thaw (SIS1), or cultured overnight in standard plates, washed, and then transferred to the SIS (SIS2). Function was assessed by determining glucose-stimulated release of insulin, which was measured by radioimmunoassay. Analysis of basal insulin secretion showed time and treatment to be significantly different (P = .0043 and P = .0123, respectively) but without an interaction (P > .05). The two SIS treatments were not significantly different (P > .05); however, both SIS1 and SIS2 were significantly different from controls (P = .0108 and P = .0420, respectively). Similar results were obtained for stimulation indices; time and treatment were significantly different (P = .0161 and P = .0264, respectively) but not an interaction (P gt; .05). The two SIS treatments were not significantly different (P = .05); however, both SIS 1 and SIS2 differed from controls (P = .0248 and P = .0407, respectively). The results indicate that SIS enables frozen-thawed islets to exhibit superior post-thaw function compared with a non-SIS-supported condition.",
author = "Woods, {E. J.} and Walsh, {C. M.} and Sidner, {R. A.} and Zieger, {M. A J} and S. Mullin and Lakey, {J. R T} and Camillo Ricordi and Critser, {J. K.}",
year = "2004",
month = "5",
day = "1",
doi = "10.1016/j.transproceed.2004.05.022",
language = "English",
volume = "36",
pages = "1139--1142",
journal = "Transplantation Proceedings",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "4",

}

TY - JOUR

T1 - Enhanced recovery of cryopreserved islets using SIS

AU - Woods, E. J.

AU - Walsh, C. M.

AU - Sidner, R. A.

AU - Zieger, M. A J

AU - Mullin, S.

AU - Lakey, J. R T

AU - Ricordi, Camillo

AU - Critser, J. K.

PY - 2004/5/1

Y1 - 2004/5/1

N2 - Although cryopreservation of pancreatic islets would add flexibility to transplantation, the recoveries are only 60% to 90% and function is decreased. Islets are multicellular structures ∼50 to 250 μm in diameter organized into a network of cells and vascular channels. Due to this complexity, islets are more susceptible to damage during cryopreservation than an individual cell. This study investigated porcine small intestinal submucosa (SIS) as a matrix to support islets recovery and function post-thaw. Groups of frozen/thawed human islets (150 IE/condition; n = 4 preparations) were cultured for 5 weeks in plates containing noncoated Biopore membrane inserts alone or inserts covered with SIS. Islets were placed directly on the insert post-thaw (SIS1), or cultured overnight in standard plates, washed, and then transferred to the SIS (SIS2). Function was assessed by determining glucose-stimulated release of insulin, which was measured by radioimmunoassay. Analysis of basal insulin secretion showed time and treatment to be significantly different (P = .0043 and P = .0123, respectively) but without an interaction (P > .05). The two SIS treatments were not significantly different (P > .05); however, both SIS1 and SIS2 were significantly different from controls (P = .0108 and P = .0420, respectively). Similar results were obtained for stimulation indices; time and treatment were significantly different (P = .0161 and P = .0264, respectively) but not an interaction (P gt; .05). The two SIS treatments were not significantly different (P = .05); however, both SIS 1 and SIS2 differed from controls (P = .0248 and P = .0407, respectively). The results indicate that SIS enables frozen-thawed islets to exhibit superior post-thaw function compared with a non-SIS-supported condition.

AB - Although cryopreservation of pancreatic islets would add flexibility to transplantation, the recoveries are only 60% to 90% and function is decreased. Islets are multicellular structures ∼50 to 250 μm in diameter organized into a network of cells and vascular channels. Due to this complexity, islets are more susceptible to damage during cryopreservation than an individual cell. This study investigated porcine small intestinal submucosa (SIS) as a matrix to support islets recovery and function post-thaw. Groups of frozen/thawed human islets (150 IE/condition; n = 4 preparations) were cultured for 5 weeks in plates containing noncoated Biopore membrane inserts alone or inserts covered with SIS. Islets were placed directly on the insert post-thaw (SIS1), or cultured overnight in standard plates, washed, and then transferred to the SIS (SIS2). Function was assessed by determining glucose-stimulated release of insulin, which was measured by radioimmunoassay. Analysis of basal insulin secretion showed time and treatment to be significantly different (P = .0043 and P = .0123, respectively) but without an interaction (P > .05). The two SIS treatments were not significantly different (P > .05); however, both SIS1 and SIS2 were significantly different from controls (P = .0108 and P = .0420, respectively). Similar results were obtained for stimulation indices; time and treatment were significantly different (P = .0161 and P = .0264, respectively) but not an interaction (P gt; .05). The two SIS treatments were not significantly different (P = .05); however, both SIS 1 and SIS2 differed from controls (P = .0248 and P = .0407, respectively). The results indicate that SIS enables frozen-thawed islets to exhibit superior post-thaw function compared with a non-SIS-supported condition.

UR - http://www.scopus.com/inward/record.url?scp=2942659929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942659929&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2004.05.022

DO - 10.1016/j.transproceed.2004.05.022

M3 - Article

C2 - 15194397

AN - SCOPUS:2942659929

VL - 36

SP - 1139

EP - 1142

JO - Transplantation Proceedings

JF - Transplantation Proceedings

SN - 0041-1345

IS - 4

ER -