Abstract
Bacterial whole cell-based biosensors have been genetically engineered to achieve selective and reliable detection of a wide range of hazardous chemicals. Although whole-cell biosensors demonstrate many advantages for field-based detection of target analytes, there are still some challenges that need to be addressed. Most notably, their often modest shelf life and need for special handling and storage make them challenging to use in situations where access to reagents, instrumentation, and expertise are limited. These problems can be circumvented by developing biosensors in Bacillus spores, which can be engineered to address all of these concerns. In its sporulated state, a whole cell-based biosensor has a remarkably long life span and is exceptionally resistant to environmental insult. When these spores are germinated for use in analytical techniques, they show no loss in performance, even after long periods of storage under harsh conditions. In this chapter, we will discuss the development and use of whole cell-based sensors, their adaptation to spore-based biosensors, their current applications, and future directions in the field.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Enzymology |
| Publisher | Academic Press Inc. |
| Pages | 51-85 |
| Number of pages | 35 |
| Volume | 589 |
| DOIs | |
| State | Published - 2017 |
Publication series
| Name | Methods in Enzymology |
|---|---|
| Volume | 589 |
| ISSN (Print) | 00766879 |
| ISSN (Electronic) | 15577988 |
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Keywords
- Bacterial endospores
- Biosensor
- Field assay
- Pollution
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Engineering Rugged Field Assays to Detect Hazardous Chemicals Using Spore-Based Bacterial Biosensors. / Wynn, Daniel; Deo, Sapna K; Daunert, Sylvia.
Methods in Enzymology. Vol. 589 Academic Press Inc., 2017. p. 51-85 (Methods in Enzymology; Vol. 589).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Engineering Rugged Field Assays to Detect Hazardous Chemicals Using Spore-Based Bacterial Biosensors
AU - Wynn, Daniel
AU - Deo, Sapna K
AU - Daunert, Sylvia
PY - 2017
Y1 - 2017
N2 - Bacterial whole cell-based biosensors have been genetically engineered to achieve selective and reliable detection of a wide range of hazardous chemicals. Although whole-cell biosensors demonstrate many advantages for field-based detection of target analytes, there are still some challenges that need to be addressed. Most notably, their often modest shelf life and need for special handling and storage make them challenging to use in situations where access to reagents, instrumentation, and expertise are limited. These problems can be circumvented by developing biosensors in Bacillus spores, which can be engineered to address all of these concerns. In its sporulated state, a whole cell-based biosensor has a remarkably long life span and is exceptionally resistant to environmental insult. When these spores are germinated for use in analytical techniques, they show no loss in performance, even after long periods of storage under harsh conditions. In this chapter, we will discuss the development and use of whole cell-based sensors, their adaptation to spore-based biosensors, their current applications, and future directions in the field.
AB - Bacterial whole cell-based biosensors have been genetically engineered to achieve selective and reliable detection of a wide range of hazardous chemicals. Although whole-cell biosensors demonstrate many advantages for field-based detection of target analytes, there are still some challenges that need to be addressed. Most notably, their often modest shelf life and need for special handling and storage make them challenging to use in situations where access to reagents, instrumentation, and expertise are limited. These problems can be circumvented by developing biosensors in Bacillus spores, which can be engineered to address all of these concerns. In its sporulated state, a whole cell-based biosensor has a remarkably long life span and is exceptionally resistant to environmental insult. When these spores are germinated for use in analytical techniques, they show no loss in performance, even after long periods of storage under harsh conditions. In this chapter, we will discuss the development and use of whole cell-based sensors, their adaptation to spore-based biosensors, their current applications, and future directions in the field.
KW - Bacterial endospores
KW - Biosensor
KW - Field assay
KW - Pollution
UR - http://www.scopus.com/inward/record.url?scp=85014771815&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014771815&partnerID=8YFLogxK
U2 - 10.1016/bs.mie.2017.02.005
DO - 10.1016/bs.mie.2017.02.005
M3 - Chapter
C2 - 28336074
AN - SCOPUS:85014771815
VL - 589
T3 - Methods in Enzymology
SP - 51
EP - 85
BT - Methods in Enzymology
PB - Academic Press Inc.
ER -