Engineering of human cartilage rods

Potential application for penile prostheses

Byung Soo Kim, James J. Yoo, Anthony Atala

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Purpose: Natural penile prostheses created from the patient's own cells may eliminate the biocompatibility risks associated with artificial prostheses. We previously demonstrated that autologous cartilaginous rods could be created in animal corpus cavernosum as penile prostheses in situ by transplanting autologous chondrocytes on biodegradable polymer scaffolds. In the present study we investigated the possibility of engineering human cartilage rods for potential use as penile prostheses. Materials and Methods: Chondrocytes isolated from human ear were seeded on rod shaped biodegradable polymer scaffolds (1.2 cm. in diameter, 6.0 cm. long). The seeded scaffolds were maintained in stirred bioreactors for 1 month. Subsequently, the seeded scaffolds were implanted subcutaneously into athymic rats. The specimens were retrieved 2 months after implantation, and histological, structural and mechanical properties were analyzed. The mechanical properties of the engineered prostheses were compared to those of silicone prostheses. Results: Human chondrocytes seeded onto polymer scaffolds formed milk-white cartilaginous rods of the same size as the initial implants. Histological analyses using hematoxylin and eosin, toluidine blue and alcian blue showed mature and well-formed chrondrocytes in the retrieved implants. The engineered human cartilaginous rods were flexible, elastic and able to withstand high degrees of compressive forces. The mechanical properties were comparable to those of commercially available silicone prostheses. Conclusions: Transplantation of chrondrocytes isolated from human ear on polymer scaffolds resulted in the formation of human cartilage rods with the appropriate mechanical properties required for use as penile prostheses. This study demonstrates the feasibility of creating human cartilage rods with a large dimension. This technology may be useful for patients who need penile reconstruction.

Original languageEnglish
Pages (from-to)1794-1797
Number of pages4
JournalJournal of Urology
Volume168
Issue number4 II
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Penile Prosthesis
Human Engineering
Cartilage
Prostheses and Implants
Polymers
Chondrocytes
Silicones
Ear
Nude Rats
Tolonium Chloride
Alcian Blue
Feasibility Studies
Bioreactors
Hematoxylin
Eosine Yellowish-(YS)
Milk
Transplantation
Technology

Keywords

  • Cartilage
  • Chondrocytes
  • Penile prosthesis

ASJC Scopus subject areas

  • Urology

Cite this

Kim, B. S., Yoo, J. J., & Atala, A. (2002). Engineering of human cartilage rods: Potential application for penile prostheses. Journal of Urology, 168(4 II), 1794-1797.

Engineering of human cartilage rods : Potential application for penile prostheses. / Kim, Byung Soo; Yoo, James J.; Atala, Anthony.

In: Journal of Urology, Vol. 168, No. 4 II, 01.10.2002, p. 1794-1797.

Research output: Contribution to journalArticle

Kim, BS, Yoo, JJ & Atala, A 2002, 'Engineering of human cartilage rods: Potential application for penile prostheses', Journal of Urology, vol. 168, no. 4 II, pp. 1794-1797.
Kim, Byung Soo ; Yoo, James J. ; Atala, Anthony. / Engineering of human cartilage rods : Potential application for penile prostheses. In: Journal of Urology. 2002 ; Vol. 168, No. 4 II. pp. 1794-1797.
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