Engineering of blood vessels from acellular collagen matrices coated with human endothelial cells

Gilad E. Amiel, Makoto Komura, Oz Shapira, James J. Yoo, Saami Yazdani, Joel Berry, Sunjay Kaushal, Joyce Bischoff, Anthony Atala, Shay Soker

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Small-caliber synthetic grafts used for coronary bypass artery grafting are compromised by thrombogenicity and accelerated intimal thickening, resulting in early graft occlusion. Herein we describe the fabrication and physical properties of small-caliber blood vessels using decellularized porcine aortic segments. These vessels were further coated with human saphenous vein endothelial cells (HSVECs) for future clinical applications. Chemical staining of decellularized vessels showed that they preserved their native matrix architecture, including several collagen layers in between internal and external elastin layers. The burst pressure for the decellularized vessels was higher than 1,000 mmHg. HSVECs, seeded on the luminal side, adhered to the matrix and formed a uniform monolayer. HSVEC-seeded vessels produced prostaglandin I 2 and released vasoactive agents in response to the calcium ionophore A23187. These results show that engineered blood vessels coated with the host endothelial cells possess morphologic and functional characteristics of human small-caliber vessels. Tissue-engineered vessels may potentially be useful clinically as vascular grafts.

Original languageEnglish
Pages (from-to)2355-2365
Number of pages11
JournalTissue Engineering
Volume12
Issue number8
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

Fingerprint

Endothelial cells
Blood vessels
Collagen
Blood Vessels
Saphenous Vein
Endothelial Cells
Grafts
Transplants
Tunica Intima
Ionophores
Elastin
Calcium Ionophores
Calcimycin
Epoprostenol
Coronary Artery Bypass
Calcium
Monolayers
Swine
Physical properties
Tissue

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Amiel, G. E., Komura, M., Shapira, O., Yoo, J. J., Yazdani, S., Berry, J., ... Soker, S. (2006). Engineering of blood vessels from acellular collagen matrices coated with human endothelial cells. Tissue Engineering, 12(8), 2355-2365. https://doi.org/10.1089/ten.2006.12.2355

Engineering of blood vessels from acellular collagen matrices coated with human endothelial cells. / Amiel, Gilad E.; Komura, Makoto; Shapira, Oz; Yoo, James J.; Yazdani, Saami; Berry, Joel; Kaushal, Sunjay; Bischoff, Joyce; Atala, Anthony; Soker, Shay.

In: Tissue Engineering, Vol. 12, No. 8, 01.08.2006, p. 2355-2365.

Research output: Contribution to journalArticle

Amiel, GE, Komura, M, Shapira, O, Yoo, JJ, Yazdani, S, Berry, J, Kaushal, S, Bischoff, J, Atala, A & Soker, S 2006, 'Engineering of blood vessels from acellular collagen matrices coated with human endothelial cells', Tissue Engineering, vol. 12, no. 8, pp. 2355-2365. https://doi.org/10.1089/ten.2006.12.2355
Amiel, Gilad E. ; Komura, Makoto ; Shapira, Oz ; Yoo, James J. ; Yazdani, Saami ; Berry, Joel ; Kaushal, Sunjay ; Bischoff, Joyce ; Atala, Anthony ; Soker, Shay. / Engineering of blood vessels from acellular collagen matrices coated with human endothelial cells. In: Tissue Engineering. 2006 ; Vol. 12, No. 8. pp. 2355-2365.
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