Engineered microenvironments for maturation of stem cell derived cardiac myocytes

Rachel R. Besser, Matthew Ishahak, Vera Mayo, Daniel Carbonero, Isabella Claure, Ashutosh Agarwal

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Through the use of stem cell-derived cardiac myocytes, tissue-engineered human myocardial constructs are poised for modeling normal and diseased physiology of the heart, as well as discovery of novel drugs and therapeutic targets in a human relevant manner. This review highlights the recent bioengineering efforts to recapitulate microenvironmental cues to further the maturation state of newly differentiated cardiac myocytes. These techniques include long-term culture, co-culture, exposure to mechanical stimuli, 3D culture, cell-matrix interactions, and electrical stimulation. Each of these methods has produced various degrees of maturation; however, a standardized measure for cardiomyocyte maturation is not yet widely accepted by the scientific community.

Original languageEnglish (US)
Pages (from-to)124-140
Number of pages17
Issue number1
StatePublished - 2018


  • Cardiac Myocytes
  • Differentiation
  • Maturation
  • Stem cells
  • Tissue Engineering

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)


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