Energy transfer in light-adapted photosynthetic membranes

From active to saturated photosynthesis

Francesca Fassioli, Alexandra Olaya-Castro, Simon Scheuring, James N. Sturgis, Neil F Johnson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In bacterial photosynthesis light-harvesting complexes, LH2 and LH1 absorb sunlight energy and deliver it to reaction centers (RCs) with extraordinarily high efficiency. Submolecular resolution images have revealed that both the LH2:LH1 ratio, and the architecture of the photosynthetic membrane itself, adapt to light intensity. We investigate the functional implications of structural adaptations in the energy transfer performance in natural in vivo low- and high-light-adapted membrane architectures of Rhodospirillum photometricum. A model is presented to describe excitation migration across the full range of light intensities that cover states from active photosynthesis, where all RCs are available for charge separation, to saturated photosynthesis where all RCs are unavailable. Our study outlines three key findings. First, there is a critical light-energy density, below which the low-light adapted membrane is more efficient at absorbing photons and generating a charge separation at RCs, than the high-light-adapted membrane. Second, connectivity of core complexes is similar in both membranes, suggesting that, despite different growth conditions, a preferred transfer pathway is through core-core contacts. Third, there may be minimal subareas on the membrane which, containing the same LH2:LH1 ratio, behave as minimal functional units as far as excitation transfer efficiency is concerned.

Original languageEnglish (US)
Pages (from-to)2464-2473
Number of pages10
JournalBiophysical Journal
Volume97
Issue number9
DOIs
StatePublished - Nov 4 2009

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Energy Transfer
Photosynthesis
Light
Membranes
Rhodospirillum
Sunlight
Photons
Growth

ASJC Scopus subject areas

  • Biophysics

Cite this

Fassioli, F., Olaya-Castro, A., Scheuring, S., Sturgis, J. N., & Johnson, N. F. (2009). Energy transfer in light-adapted photosynthetic membranes: From active to saturated photosynthesis. Biophysical Journal, 97(9), 2464-2473. https://doi.org/10.1016/j.bpj.2009.08.033

Energy transfer in light-adapted photosynthetic membranes : From active to saturated photosynthesis. / Fassioli, Francesca; Olaya-Castro, Alexandra; Scheuring, Simon; Sturgis, James N.; Johnson, Neil F.

In: Biophysical Journal, Vol. 97, No. 9, 04.11.2009, p. 2464-2473.

Research output: Contribution to journalArticle

Fassioli, F, Olaya-Castro, A, Scheuring, S, Sturgis, JN & Johnson, NF 2009, 'Energy transfer in light-adapted photosynthetic membranes: From active to saturated photosynthesis', Biophysical Journal, vol. 97, no. 9, pp. 2464-2473. https://doi.org/10.1016/j.bpj.2009.08.033
Fassioli, Francesca ; Olaya-Castro, Alexandra ; Scheuring, Simon ; Sturgis, James N. ; Johnson, Neil F. / Energy transfer in light-adapted photosynthetic membranes : From active to saturated photosynthesis. In: Biophysical Journal. 2009 ; Vol. 97, No. 9. pp. 2464-2473.
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