Abstract
A novel fluorescence method has been developed which should have general applicability for the study of protein-nucleic acid interactions. The association between proteins covalently labeled with dansyl chloride and DNA noncovalently labeled with acridine orange can be studied by monitoring the transfer of fluorescent energy from the donor (the dansyl label) to the acceptor (acridine orange). This method has been illustrated in model fluorescence studies which yield estimates of the combining ratio of histone VIIIS (Sigma, arginine rich) with DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 208-213 |
| Number of pages | 6 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 180 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 15 1977 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Energy transfer : A general method for the study of protein-nucleic acid interactions. / Taylor, Ronald P.; Riley, Richard L; Weber, Daniel.
In: Archives of Biochemistry and Biophysics, Vol. 180, No. 1, 15.04.1977, p. 208-213.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Energy transfer
T2 - A general method for the study of protein-nucleic acid interactions
AU - Taylor, Ronald P.
AU - Riley, Richard L
AU - Weber, Daniel
PY - 1977/4/15
Y1 - 1977/4/15
N2 - A novel fluorescence method has been developed which should have general applicability for the study of protein-nucleic acid interactions. The association between proteins covalently labeled with dansyl chloride and DNA noncovalently labeled with acridine orange can be studied by monitoring the transfer of fluorescent energy from the donor (the dansyl label) to the acceptor (acridine orange). This method has been illustrated in model fluorescence studies which yield estimates of the combining ratio of histone VIIIS (Sigma, arginine rich) with DNA.
AB - A novel fluorescence method has been developed which should have general applicability for the study of protein-nucleic acid interactions. The association between proteins covalently labeled with dansyl chloride and DNA noncovalently labeled with acridine orange can be studied by monitoring the transfer of fluorescent energy from the donor (the dansyl label) to the acceptor (acridine orange). This method has been illustrated in model fluorescence studies which yield estimates of the combining ratio of histone VIIIS (Sigma, arginine rich) with DNA.
UR - http://www.scopus.com/inward/record.url?scp=0017332336&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017332336&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(77)90027-3
DO - 10.1016/0003-9861(77)90027-3
M3 - Article
C2 - 856043
AN - SCOPUS:0017332336
VL - 180
SP - 208
EP - 213
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 1
ER -