Energy transfer: A general method for the study of protein-nucleic acid interactions

Ronald P. Taylor; Richard L. Riley; Daniel Weber

doi:10.1016/0003-9861(77)90027-3

Energy transfer: A general method for the study of protein-nucleic acid interactions

Ronald P. Taylor, Richard L. Riley, Daniel Weber

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A novel fluorescence method has been developed which should have general applicability for the study of protein-nucleic acid interactions. The association between proteins covalently labeled with dansyl chloride and DNA noncovalently labeled with acridine orange can be studied by monitoring the transfer of fluorescent energy from the donor (the dansyl label) to the acceptor (acridine orange). This method has been illustrated in model fluorescence studies which yield estimates of the combining ratio of histone VIIIS (Sigma, arginine rich) with DNA.

Original language	English (US)
Pages (from-to)	208-213
Number of pages	6
Journal	Archives of Biochemistry and Biophysics
Volume	180
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(77)90027-3
State	Published - Apr 15 1977
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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abstract = "A novel fluorescence method has been developed which should have general applicability for the study of protein-nucleic acid interactions. The association between proteins covalently labeled with dansyl chloride and DNA noncovalently labeled with acridine orange can be studied by monitoring the transfer of fluorescent energy from the donor (the dansyl label) to the acceptor (acridine orange). This method has been illustrated in model fluorescence studies which yield estimates of the combining ratio of histone VIIIS (Sigma, arginine rich) with DNA.",

author = "Taylor, {Ronald P.} and Riley, {Richard L.} and Daniel Weber",

note = "Funding Information: I Supported by NIH Grant No. AI-1173503. {\textquoteright} Research Career Development Awardee National Institutes of Health, No. AI-00062-02. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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AU - Riley, Richard L.

AU - Weber, Daniel

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N2 - A novel fluorescence method has been developed which should have general applicability for the study of protein-nucleic acid interactions. The association between proteins covalently labeled with dansyl chloride and DNA noncovalently labeled with acridine orange can be studied by monitoring the transfer of fluorescent energy from the donor (the dansyl label) to the acceptor (acridine orange). This method has been illustrated in model fluorescence studies which yield estimates of the combining ratio of histone VIIIS (Sigma, arginine rich) with DNA.

AB - A novel fluorescence method has been developed which should have general applicability for the study of protein-nucleic acid interactions. The association between proteins covalently labeled with dansyl chloride and DNA noncovalently labeled with acridine orange can be studied by monitoring the transfer of fluorescent energy from the donor (the dansyl label) to the acceptor (acridine orange). This method has been illustrated in model fluorescence studies which yield estimates of the combining ratio of histone VIIIS (Sigma, arginine rich) with DNA.

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Energy transfer: A general method for the study of protein-nucleic acid interactions

Abstract

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