Energy transfer: A general method for the study of protein-nucleic acid interactions

Ronald P. Taylor, Richard L Riley, Daniel Weber

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel fluorescence method has been developed which should have general applicability for the study of protein-nucleic acid interactions. The association between proteins covalently labeled with dansyl chloride and DNA noncovalently labeled with acridine orange can be studied by monitoring the transfer of fluorescent energy from the donor (the dansyl label) to the acceptor (acridine orange). This method has been illustrated in model fluorescence studies which yield estimates of the combining ratio of histone VIIIS (Sigma, arginine rich) with DNA.

Original languageEnglish
Pages (from-to)208-213
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume180
Issue number1
DOIs
StatePublished - Apr 15 1977
Externally publishedYes

Fingerprint

Acridine Orange
Energy Transfer
Energy transfer
Nucleic Acids
Fluorescence
DNA
Histones
Arginine
Labels
Proteins
Monitoring
dansyl chloride

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Energy transfer : A general method for the study of protein-nucleic acid interactions. / Taylor, Ronald P.; Riley, Richard L; Weber, Daniel.

In: Archives of Biochemistry and Biophysics, Vol. 180, No. 1, 15.04.1977, p. 208-213.

Research output: Contribution to journalArticle

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