Energy metabolism of intervertebral disc under mechanical loading

Chong Wang, Silvia Gonzales, Howard Levene, Weiyong Gu, Chun-Yuh Huang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Intervertebral disc (IVD) degeneration is closely associated with low back pain (LBP), which is a major health concern in the U.S. Cellular biosynthesis of extracellular matrix (ECM), which is important for maintaining tissue integrity and preventing tissue degeneration, is an energy demanding process. Due to impaired nutrient support in avascular IVD, adenosine triphosphate (ATP) supply could be a limiting factor for maintaining normal ECM synthesis. Therefore, the objective of this study was to investigate the energy metabolism in the annulus fibrosus (AF) and nucleus pulposus (NP) of porcine IVD under static and dynamic compressions. Under compression, pH decreased and the contents of lactate and ATP increased significantly in both AF and NP regions, suggesting that compression can promote ATP production via glycolysis and reduce pH by increasing lactate accumulation. A high level of extracellular ATP content was detected in the NP region and regulated by compressive loading. Since ATP can serve not only as an intra-cellular energy currency, but also as a regulator of a variety of cellular activities extracellularly through the purinergic signaling pathway, our findings suggest that compression-mediated ATP metabolism could be a novel mechanobiological pathway for regulating IVD metabolism.

Original languageEnglish
Pages (from-to)1733-1738
Number of pages6
JournalJournal of Orthopaedic Research
Volume31
Issue number11
DOIs
StatePublished - Nov 1 2013

Fingerprint

Intervertebral Disc
Energy Metabolism
Adenosine Triphosphate
Extracellular Matrix
Lactic Acid
Intervertebral Disc Degeneration
Glycolysis
Low Back Pain
Swine
Food
Health
Nucleus Pulposus

Keywords

  • ATP
  • energy metabolism
  • IVD
  • lactate
  • mechanical loading

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Energy metabolism of intervertebral disc under mechanical loading. / Wang, Chong; Gonzales, Silvia; Levene, Howard; Gu, Weiyong; Huang, Chun-Yuh.

In: Journal of Orthopaedic Research, Vol. 31, No. 11, 01.11.2013, p. 1733-1738.

Research output: Contribution to journalArticle

@article{3884f4a267d24e5c9f98a7907490ecca,
title = "Energy metabolism of intervertebral disc under mechanical loading",
abstract = "Intervertebral disc (IVD) degeneration is closely associated with low back pain (LBP), which is a major health concern in the U.S. Cellular biosynthesis of extracellular matrix (ECM), which is important for maintaining tissue integrity and preventing tissue degeneration, is an energy demanding process. Due to impaired nutrient support in avascular IVD, adenosine triphosphate (ATP) supply could be a limiting factor for maintaining normal ECM synthesis. Therefore, the objective of this study was to investigate the energy metabolism in the annulus fibrosus (AF) and nucleus pulposus (NP) of porcine IVD under static and dynamic compressions. Under compression, pH decreased and the contents of lactate and ATP increased significantly in both AF and NP regions, suggesting that compression can promote ATP production via glycolysis and reduce pH by increasing lactate accumulation. A high level of extracellular ATP content was detected in the NP region and regulated by compressive loading. Since ATP can serve not only as an intra-cellular energy currency, but also as a regulator of a variety of cellular activities extracellularly through the purinergic signaling pathway, our findings suggest that compression-mediated ATP metabolism could be a novel mechanobiological pathway for regulating IVD metabolism.",
keywords = "ATP, energy metabolism, IVD, lactate, mechanical loading",
author = "Chong Wang and Silvia Gonzales and Howard Levene and Weiyong Gu and Chun-Yuh Huang",
year = "2013",
month = "11",
day = "1",
doi = "10.1002/jor.22436",
language = "English",
volume = "31",
pages = "1733--1738",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

TY - JOUR

T1 - Energy metabolism of intervertebral disc under mechanical loading

AU - Wang, Chong

AU - Gonzales, Silvia

AU - Levene, Howard

AU - Gu, Weiyong

AU - Huang, Chun-Yuh

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Intervertebral disc (IVD) degeneration is closely associated with low back pain (LBP), which is a major health concern in the U.S. Cellular biosynthesis of extracellular matrix (ECM), which is important for maintaining tissue integrity and preventing tissue degeneration, is an energy demanding process. Due to impaired nutrient support in avascular IVD, adenosine triphosphate (ATP) supply could be a limiting factor for maintaining normal ECM synthesis. Therefore, the objective of this study was to investigate the energy metabolism in the annulus fibrosus (AF) and nucleus pulposus (NP) of porcine IVD under static and dynamic compressions. Under compression, pH decreased and the contents of lactate and ATP increased significantly in both AF and NP regions, suggesting that compression can promote ATP production via glycolysis and reduce pH by increasing lactate accumulation. A high level of extracellular ATP content was detected in the NP region and regulated by compressive loading. Since ATP can serve not only as an intra-cellular energy currency, but also as a regulator of a variety of cellular activities extracellularly through the purinergic signaling pathway, our findings suggest that compression-mediated ATP metabolism could be a novel mechanobiological pathway for regulating IVD metabolism.

AB - Intervertebral disc (IVD) degeneration is closely associated with low back pain (LBP), which is a major health concern in the U.S. Cellular biosynthesis of extracellular matrix (ECM), which is important for maintaining tissue integrity and preventing tissue degeneration, is an energy demanding process. Due to impaired nutrient support in avascular IVD, adenosine triphosphate (ATP) supply could be a limiting factor for maintaining normal ECM synthesis. Therefore, the objective of this study was to investigate the energy metabolism in the annulus fibrosus (AF) and nucleus pulposus (NP) of porcine IVD under static and dynamic compressions. Under compression, pH decreased and the contents of lactate and ATP increased significantly in both AF and NP regions, suggesting that compression can promote ATP production via glycolysis and reduce pH by increasing lactate accumulation. A high level of extracellular ATP content was detected in the NP region and regulated by compressive loading. Since ATP can serve not only as an intra-cellular energy currency, but also as a regulator of a variety of cellular activities extracellularly through the purinergic signaling pathway, our findings suggest that compression-mediated ATP metabolism could be a novel mechanobiological pathway for regulating IVD metabolism.

KW - ATP

KW - energy metabolism

KW - IVD

KW - lactate

KW - mechanical loading

UR - http://www.scopus.com/inward/record.url?scp=84884905164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884905164&partnerID=8YFLogxK

U2 - 10.1002/jor.22436

DO - 10.1002/jor.22436

M3 - Article

C2 - 23843186

AN - SCOPUS:84884905164

VL - 31

SP - 1733

EP - 1738

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 11

ER -