Endoxin testing with limulus amoebocyte lysate in a radiopharmaceutical containing chelated metallic radionuclides and chelating agents

M. K. Dewanjee, S. C. Chowdhury, T. Herold, Aldo N Serafini, George N Sfakianakis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The gel formation by endotoxin with limulus amoebocyte lysate (LAL) solution requires the presence of free calcium ions. The chelating agents and radiopharmaceuticals prepared from the chelating agents always reduce the available free calcium levels present in the kits used for the testing of apyrogenicity and thus inhibit gel formation of LAL with E. coli endotoxin (ET). This inhibition of gel formation could be reversed by the addition of excess free calcium ions or the excessive dilution of radiopharmaceuticals and chelating agents. The tests of positive control (0.2 ml ET units and LAL), inhibition control (O.1 ml sample, 0.1 ml ET and LAL), and negative control (0.1 ml sterile saline and LAL) were carried out with the fresh preparation (0.1 ml) of samples (triplicate), tropolone, ACD anticoagulant, and 99mTc-labeled radiopharmaceuticals. In the Ca-supplemented tests, 0.1 ml of 60 mM sterile calcium chloride solution was added to the test solutions and incubated for 60 min at 37° centigrade. The results of gel formation (+ve and -ve) and normalization of inhibition control tests with Ca-supplement indicate that commercial LAL kits need extra calcium ions for the correct testing of the apyrogenicity of chelate-containing radiopharmaceuticals and chelating agents.

Original languageEnglish
Pages (from-to)243-245
Number of pages3
JournalJournal of Nuclear Medicine
Volume31
Issue number2
StatePublished - Jan 1 1990

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Horseshoe Crabs
Radiopharmaceuticals
Chelating Agents
Radioisotopes
Endotoxins
Gels
Calcium
Ions
Tropolone
Calcium Chloride
Anticoagulants
digoxin-like factors
endotoxin binding proteins
Escherichia coli

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Endoxin testing with limulus amoebocyte lysate in a radiopharmaceutical containing chelated metallic radionuclides and chelating agents. / Dewanjee, M. K.; Chowdhury, S. C.; Herold, T.; Serafini, Aldo N; Sfakianakis, George N.

In: Journal of Nuclear Medicine, Vol. 31, No. 2, 01.01.1990, p. 243-245.

Research output: Contribution to journalArticle

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