Endothelialization of heart valve matrix using a computer-assisted pulsatile bioreactor

Dong Joon Lee, Julie Steen, James E. Jordan, Edward H. Kincaid, Neal D. Kon, Anthony Atala, Joel Berry, James J. Yoo

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Although calcification remains as the main clinical concern associated with bioprosthetic heart valve replacement surgery, there is evidence that tissue deterioration leads to thromboembolism. In such instances, measures that prevent thrombosis may be beneficial. To minimize thrombosis, endothelialization of the valve surface before implantation has been proposed to facilitate coverage. In this study we aimed to define the optimal flow parameters for the endothelialization of decellularized heart valves using endothelial progenitor cell (EPC)-derived endothelial cells (ECs). We assessed the thrombogenic characteristics of the endothelialized heart valve surface using a bioreactor. EPC-derived ECs were seeded on decellularized porcine valve scaffolds. A computer-controlled bioreactor system was used to determine the optimal flow rates. Successful endothelialization was achieved by preconditioning the cell-seeded valves with stepwise increases in volume flow rate up to 2aL/min for 7 days. We show that decellularized valve scaffolds seeded with EPC-derived ECs improved the anti-thrombotic properties of the valve, whereas the scaffolds without ECs escalated the coagulation process. This study demonstrates that preconditioning of ECs seeded on valve matrices using a bioreactor system is necessary for achieving uniform endothelialization of valve scaffolds, which may reduce thrombotic activity after implantation in vivo.

Original languageEnglish
Pages (from-to)807-814
Number of pages8
JournalTissue Engineering - Part A
Volume15
Issue number4
DOIs
StatePublished - Apr 1 2009
Externally publishedYes

Fingerprint

Heart Valves
Endothelial cells
Bioreactors
Endothelial Cells
Scaffolds
Thrombosis
Thromboembolism
Flow rate
Swine
Coagulation
Surgery
Deterioration
Endothelial Progenitor Cells
Tissue

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Lee, D. J., Steen, J., Jordan, J. E., Kincaid, E. H., Kon, N. D., Atala, A., ... Yoo, J. J. (2009). Endothelialization of heart valve matrix using a computer-assisted pulsatile bioreactor. Tissue Engineering - Part A, 15(4), 807-814. https://doi.org/10.1089/ten.tea.2008.0250

Endothelialization of heart valve matrix using a computer-assisted pulsatile bioreactor. / Lee, Dong Joon; Steen, Julie; Jordan, James E.; Kincaid, Edward H.; Kon, Neal D.; Atala, Anthony; Berry, Joel; Yoo, James J.

In: Tissue Engineering - Part A, Vol. 15, No. 4, 01.04.2009, p. 807-814.

Research output: Contribution to journalArticle

Lee, DJ, Steen, J, Jordan, JE, Kincaid, EH, Kon, ND, Atala, A, Berry, J & Yoo, JJ 2009, 'Endothelialization of heart valve matrix using a computer-assisted pulsatile bioreactor', Tissue Engineering - Part A, vol. 15, no. 4, pp. 807-814. https://doi.org/10.1089/ten.tea.2008.0250
Lee, Dong Joon ; Steen, Julie ; Jordan, James E. ; Kincaid, Edward H. ; Kon, Neal D. ; Atala, Anthony ; Berry, Joel ; Yoo, James J. / Endothelialization of heart valve matrix using a computer-assisted pulsatile bioreactor. In: Tissue Engineering - Part A. 2009 ; Vol. 15, No. 4. pp. 807-814.
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