Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis

Joaquin J Jimenez, Wenche Jy, Lucia M. Mauro, Carl Soderland, Lawrence L. Horstman, Yeon Ahn

Research output: Contribution to journalArticle

401 Citations (Scopus)

Abstract

Background: Endothelial cells (EC) shed endothelial microparticles (EMP) in activation and apoptosis. Objectives: We compared the antigenic expression of EMP species released during activation as compared to apoptosis, in three cell lines. Methods: EC from renal and brain microvascular (MiVEC) and coronary macrovascular (MaVEC) origin were incubated with TNF-α to induce activation, or deprived of growth factors to induce apoptosis. Antigens expressed on EMP and EC were assayed flow cytometrically and included constitutive markers (CD31, CD51/61, CD105), inducible markers (CD54, CD62E and CD106), and annexin V binding. Results: It was found that in apoptosis, constitutive markers in EMP were markedly increased (CD31>CD105), with a concomitant decrease in expression in EC. Annexin V EC surface binding and annexin V+ EMP were more sharply increased in apoptosis than in activation. In contrast, in activation, inducible markers in EMP were markedly increased in both EMP and EC (CD62E>CD54>CD106). Coronary MaVEC released significantly less EMP than MiVEC. Conclusion: EC release qualitatively and quantitatively distinct EMP during activation compared to apoptosis. Analysis of EMP phenotypic signatures may provide clinically useful information on the status of the endothelium.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalThrombosis Research
Volume109
Issue number4
DOIs
StatePublished - Feb 15 2003

Fingerprint

Endothelial Cells
Apoptosis
Annexin A5
Endothelium
Intercellular Signaling Peptides and Proteins
Kidney
Antigens
Cell Line
Brain

Keywords

  • Activation
  • Adhesion molecules
  • Apoptosis
  • Endothelial cells
  • Endothelial microparticles
  • Phenotype

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. / Jimenez, Joaquin J; Jy, Wenche; Mauro, Lucia M.; Soderland, Carl; Horstman, Lawrence L.; Ahn, Yeon.

In: Thrombosis Research, Vol. 109, No. 4, 15.02.2003, p. 175-180.

Research output: Contribution to journalArticle

Jimenez, Joaquin J ; Jy, Wenche ; Mauro, Lucia M. ; Soderland, Carl ; Horstman, Lawrence L. ; Ahn, Yeon. / Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. In: Thrombosis Research. 2003 ; Vol. 109, No. 4. pp. 175-180.
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