Abstract
Background: Endothelial cells (EC) shed endothelial microparticles (EMP) in activation and apoptosis. Objectives: We compared the antigenic expression of EMP species released during activation as compared to apoptosis, in three cell lines. Methods: EC from renal and brain microvascular (MiVEC) and coronary macrovascular (MaVEC) origin were incubated with TNF-α to induce activation, or deprived of growth factors to induce apoptosis. Antigens expressed on EMP and EC were assayed flow cytometrically and included constitutive markers (CD31, CD51/61, CD105), inducible markers (CD54, CD62E and CD106), and annexin V binding. Results: It was found that in apoptosis, constitutive markers in EMP were markedly increased (CD31>CD105), with a concomitant decrease in expression in EC. Annexin V EC surface binding and annexin V+ EMP were more sharply increased in apoptosis than in activation. In contrast, in activation, inducible markers in EMP were markedly increased in both EMP and EC (CD62E>CD54>CD106). Coronary MaVEC released significantly less EMP than MiVEC. Conclusion: EC release qualitatively and quantitatively distinct EMP during activation compared to apoptosis. Analysis of EMP phenotypic signatures may provide clinically useful information on the status of the endothelium.
| Original language | English |
|---|---|
| Pages (from-to) | 175-180 |
| Number of pages | 6 |
| Journal | Thrombosis Research |
| Volume | 109 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 15 2003 |
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Keywords
- Activation
- Adhesion molecules
- Apoptosis
- Endothelial cells
- Endothelial microparticles
- Phenotype
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Hematology
Cite this
Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. / Jimenez, Joaquin J; Jy, Wenche; Mauro, Lucia M.; Soderland, Carl; Horstman, Lawrence L.; Ahn, Yeon.
In: Thrombosis Research, Vol. 109, No. 4, 15.02.2003, p. 175-180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis
AU - Jimenez, Joaquin J
AU - Jy, Wenche
AU - Mauro, Lucia M.
AU - Soderland, Carl
AU - Horstman, Lawrence L.
AU - Ahn, Yeon
PY - 2003/2/15
Y1 - 2003/2/15
N2 - Background: Endothelial cells (EC) shed endothelial microparticles (EMP) in activation and apoptosis. Objectives: We compared the antigenic expression of EMP species released during activation as compared to apoptosis, in three cell lines. Methods: EC from renal and brain microvascular (MiVEC) and coronary macrovascular (MaVEC) origin were incubated with TNF-α to induce activation, or deprived of growth factors to induce apoptosis. Antigens expressed on EMP and EC were assayed flow cytometrically and included constitutive markers (CD31, CD51/61, CD105), inducible markers (CD54, CD62E and CD106), and annexin V binding. Results: It was found that in apoptosis, constitutive markers in EMP were markedly increased (CD31>CD105), with a concomitant decrease in expression in EC. Annexin V EC surface binding and annexin V+ EMP were more sharply increased in apoptosis than in activation. In contrast, in activation, inducible markers in EMP were markedly increased in both EMP and EC (CD62E>CD54>CD106). Coronary MaVEC released significantly less EMP than MiVEC. Conclusion: EC release qualitatively and quantitatively distinct EMP during activation compared to apoptosis. Analysis of EMP phenotypic signatures may provide clinically useful information on the status of the endothelium.
AB - Background: Endothelial cells (EC) shed endothelial microparticles (EMP) in activation and apoptosis. Objectives: We compared the antigenic expression of EMP species released during activation as compared to apoptosis, in three cell lines. Methods: EC from renal and brain microvascular (MiVEC) and coronary macrovascular (MaVEC) origin were incubated with TNF-α to induce activation, or deprived of growth factors to induce apoptosis. Antigens expressed on EMP and EC were assayed flow cytometrically and included constitutive markers (CD31, CD51/61, CD105), inducible markers (CD54, CD62E and CD106), and annexin V binding. Results: It was found that in apoptosis, constitutive markers in EMP were markedly increased (CD31>CD105), with a concomitant decrease in expression in EC. Annexin V EC surface binding and annexin V+ EMP were more sharply increased in apoptosis than in activation. In contrast, in activation, inducible markers in EMP were markedly increased in both EMP and EC (CD62E>CD54>CD106). Coronary MaVEC released significantly less EMP than MiVEC. Conclusion: EC release qualitatively and quantitatively distinct EMP during activation compared to apoptosis. Analysis of EMP phenotypic signatures may provide clinically useful information on the status of the endothelium.
KW - Activation
KW - Adhesion molecules
KW - Apoptosis
KW - Endothelial cells
KW - Endothelial microparticles
KW - Phenotype
UR - http://www.scopus.com/inward/record.url?scp=0037709857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037709857&partnerID=8YFLogxK
U2 - 10.1016/S0049-3848(03)00064-1
DO - 10.1016/S0049-3848(03)00064-1
M3 - Article
C2 - 12757771
AN - SCOPUS:0037709857
VL - 109
SP - 175
EP - 180
JO - Thrombosis Research
JF - Thrombosis Research
SN - 0049-3848
IS - 4
ER -