Endothelial cell-selective materials for tissue engineering in the vascular graft via a new receptor

Jeffrey A. Hubbell, Stephen P. Massia, Neil P. Desai, Paul D. Drumheller

Research output: Contribution to journalArticle

300 Scopus citations

Abstract

We have found a novel adhesion receptor on the human endothelial cell for the peptide sequence Arg-Glu-Asp-Val (REDV), which is present in the III-CS domain of human plasma fibronectin, with a dissociation constant of 2.2 × 10-6 M and 5.8 × 106 sites/cell. When a synthetic peptide containing this sequence was immobilized on otherwise cell nonadhesive substrates, endothelial cells attached and spread but fibroblasts, vascular smooth muscle cells, and platelets did not. Endothelial monolayers on REDV were nonthrombogenic: endothelial cells attached and spread upon other receptor-binding domains of fibronectin and laminin, but with lesser degrees of specificity or with a loss of nonthrombogenicity. This approach may provide a basis for a tissue engineered vascular graft where endothelial cell attachment is desired, but not the attachment of other blood vessel wall cells and blood platelets.

Original languageEnglish (US)
Pages (from-to)568-572
Number of pages5
JournalBio/Technology
Volume9
Issue number6
DOIs
StatePublished - Jun 1991

ASJC Scopus subject areas

  • Biotechnology

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