Endothelial cell-selective materials for tissue engineering in the vascular graft via a new receptor

Jeffrey A. Hubbell, Stephen P. Massia, Neil P. Desai, Paul D. Drumheller

Research output: Contribution to journalArticle

294 Citations (Scopus)

Abstract

We have found a novel adhesion receptor on the human endothelial cell for the peptide sequence Arg-Glu-Asp-Val (REDV), which is present in the III-CS domain of human plasma fibronectin, with a dissociation constant of 2.2 × 10-6 M and 5.8 × 106 sites/cell. When a synthetic peptide containing this sequence was immobilized on otherwise cell nonadhesive substrates, endothelial cells attached and spread but fibroblasts, vascular smooth muscle cells, and platelets did not. Endothelial monolayers on REDV were nonthrombogenic: endothelial cells attached and spread upon other receptor-binding domains of fibronectin and laminin, but with lesser degrees of specificity or with a loss of nonthrombogenicity. This approach may provide a basis for a tissue engineered vascular graft where endothelial cell attachment is desired, but not the attachment of other blood vessel wall cells and blood platelets.

Original languageEnglish
Pages (from-to)568-572
Number of pages5
JournalNature Biotechnology
Volume9
Issue number6
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Endothelial cells
Tissue Engineering
Tissue engineering
Grafts
Blood Vessels
Endothelial Cells
Transplants
Platelets
Fibronectins
Peptides
Blood Platelets
Blood Vessel Prosthesis
Plasma (human)
Blood vessels
Laminin
Fibroblasts
Vascular Smooth Muscle
Cell Wall
Smooth Muscle Myocytes
Muscle

ASJC Scopus subject areas

  • Microbiology

Cite this

Hubbell, J. A., Massia, S. P., Desai, N. P., & Drumheller, P. D. (1991). Endothelial cell-selective materials for tissue engineering in the vascular graft via a new receptor. Nature Biotechnology, 9(6), 568-572.

Endothelial cell-selective materials for tissue engineering in the vascular graft via a new receptor. / Hubbell, Jeffrey A.; Massia, Stephen P.; Desai, Neil P.; Drumheller, Paul D.

In: Nature Biotechnology, Vol. 9, No. 6, 01.12.1991, p. 568-572.

Research output: Contribution to journalArticle

Hubbell, JA, Massia, SP, Desai, NP & Drumheller, PD 1991, 'Endothelial cell-selective materials for tissue engineering in the vascular graft via a new receptor', Nature Biotechnology, vol. 9, no. 6, pp. 568-572.
Hubbell, Jeffrey A. ; Massia, Stephen P. ; Desai, Neil P. ; Drumheller, Paul D. / Endothelial cell-selective materials for tissue engineering in the vascular graft via a new receptor. In: Nature Biotechnology. 1991 ; Vol. 9, No. 6. pp. 568-572.
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