Endothelial antigen presentation: Stimulation of previously activated but not naive TCR-transgenic mouse T cells

Victor L Perez Quinones, Lori Henault, Andrew H. Lichtman

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In vitro experiments have shown that endothelial cells can function as antigen-presenting cells to CD4+ T lymphocytes. The studies presented here address the question of whether naive versus previously activated CD4+ helper T cells differ in their responses to endothelial antigen presentation. TCR-transgenic mice were used as a source of naive T cells of defined antigen specificity. These cells were stimulated in vitro with antigen and splenic antigen-presenting cells to generate populations of T lymphocytes with a previously activated/memory phenotype. Two different types of mouse endothelial cells were used as antigen-presenting cells, including the SVEC4- 10 line derived from lymph node endothelium and primary murine pulmonary microvascular endothelium. Monolayer cultures of both types of endothelium were capable of antigen-dependent stimulation of previously activated TCR- transgenic CD4+ cells. In contrast, neither endothelial type could activate naive CD4+ T cells. When costimulatory signals were provided in trans by the addition of MHC-mismatched mouse spleen cells, activation of naive T cells by endothelial antigen presentation could be demonstrated. The expression of ICAM-1 or VCAM-1 on the endothelial cells was not sufficient to activate naive T cells. Furthermore, the mouse lung endothelium constitutively expresses B7-1, and therefore, the inability of endothelium to stimulate naive T cells could not be attributed to a lack of CD28-ligands. These studies suggest that the potential role of endothelial antigen presentation in immune responses is restricted to promoting responses by T cells which have previously encountered antigen presented by other antigen-presenting cells.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalCellular Immunology
Volume189
Issue number1
DOIs
StatePublished - Oct 10 1998
Externally publishedYes

Fingerprint

Antigen Presentation
Transgenic Mice
T-Lymphocytes
Endothelium
Antigen-Presenting Cells
Antigens
Endothelial Cells
Lung
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Helper-Inducer T-Lymphocytes
Spleen
Lymph Nodes
Ligands
Phenotype
Population

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Endothelial antigen presentation : Stimulation of previously activated but not naive TCR-transgenic mouse T cells. / Perez Quinones, Victor L; Henault, Lori; Lichtman, Andrew H.

In: Cellular Immunology, Vol. 189, No. 1, 10.10.1998, p. 31-40.

Research output: Contribution to journalArticle

Perez Quinones, Victor L ; Henault, Lori ; Lichtman, Andrew H. / Endothelial antigen presentation : Stimulation of previously activated but not naive TCR-transgenic mouse T cells. In: Cellular Immunology. 1998 ; Vol. 189, No. 1. pp. 31-40.
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