Endogenous methyl palmitate modulates nicotinic receptor-mediated transmission in the superior cervical ganglion

Hung Wen Lin, Chao Zong Liu, Deshou Cao, Po Yi Chen, Mei Fang Chen, Shinn Zong Lin, Mansoor Mozayan, Alex F. Chen, Louis S. Premkumar, Donald S. Torry, Tony J F Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Nitric oxide (NO) is identified as the endothelium-derived relaxing factor and a neurotransmitter with a superfusion bioassay cascade technique. By using a similar technique with rat superior cervical ganglion (SCG) as donor tissue and rabbit endothelium-denuded aortic ring as detector tissue, we report here that a vasodilator, which is more potent than NO, is released in the SCG upon field electrical stimulation (FES) or addition of nicotine. Release of this vasodilator was enhanced by arginine analogs, including Nω- nitro-L-arginine (a NO synthase inhibitor), suggesting that it is not NO. Analysis by gas chromatography/mass spectrometry identified 2 saturated fatty acids, palmitic acid methyl ester (PAME) and stearic acid methyl ester (SAME), being released from the SCG upon FES in the presence of arginine analogs. Exogenous PAME but not SAME induced significant aortic dilation (EC50 = 0.19 nM), indicating that PAME is the potent vasodilator. Release of PAME and SAME was significantly diminished in chronically decentralized SCG but not denervated SCG, suggesting the preganglionic origin. Furthermore, release of both fatty acids was calcium- and myosin light chain kinase-dependent, suggesting that both were released from axoplasmic vesicular stores. Electrophysiological studies further demonstrated that PAME but not SAME inhibited nicotine-induced inward currents in cultured SCG and the α7-nicotinic acetylcholine receptor-expressing Xenopus oocytes. Endogenous PAME appears to play a role in modulation of the autonomic ganglionic transmission and to complement the vasodilator effect of NO.

Original languageEnglish (US)
Pages (from-to)19526-19531
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number49
DOIs
StatePublished - Dec 9 2008

Fingerprint

Superior Cervical Ganglion
Nicotinic Receptors
Vasodilator Agents
Nitric Oxide
Esters
Arginine
Nicotine
Electric Stimulation
Fatty Acids
Myosin-Light-Chain Kinase
Endothelium-Dependent Relaxing Factors
Xenopus
Nitric Oxide Synthase
Biological Assay
Gas Chromatography-Mass Spectrometry
Endothelium
Oocytes
Neurotransmitter Agents
methyl palmitate
Dilatation

Keywords

  • Alpha7-nicotinic acetylcholine receptor
  • Fatty acid vasodilator
  • Palmitic and stearic acid methyl ester
  • Superfusion bioassay cascade

ASJC Scopus subject areas

  • General

Cite this

Endogenous methyl palmitate modulates nicotinic receptor-mediated transmission in the superior cervical ganglion. / Wen Lin, Hung; Liu, Chao Zong; Cao, Deshou; Chen, Po Yi; Chen, Mei Fang; Lin, Shinn Zong; Mozayan, Mansoor; Chen, Alex F.; Premkumar, Louis S.; Torry, Donald S.; Lee, Tony J F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 49, 09.12.2008, p. 19526-19531.

Research output: Contribution to journalArticle

Wen Lin, H, Liu, CZ, Cao, D, Chen, PY, Chen, MF, Lin, SZ, Mozayan, M, Chen, AF, Premkumar, LS, Torry, DS & Lee, TJF 2008, 'Endogenous methyl palmitate modulates nicotinic receptor-mediated transmission in the superior cervical ganglion', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 49, pp. 19526-19531. https://doi.org/10.1073/pnas.0810262105
Wen Lin, Hung ; Liu, Chao Zong ; Cao, Deshou ; Chen, Po Yi ; Chen, Mei Fang ; Lin, Shinn Zong ; Mozayan, Mansoor ; Chen, Alex F. ; Premkumar, Louis S. ; Torry, Donald S. ; Lee, Tony J F. / Endogenous methyl palmitate modulates nicotinic receptor-mediated transmission in the superior cervical ganglion. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 49. pp. 19526-19531.
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AU - Wen Lin, Hung

AU - Liu, Chao Zong

AU - Cao, Deshou

AU - Chen, Po Yi

AU - Chen, Mei Fang

AU - Lin, Shinn Zong

AU - Mozayan, Mansoor

AU - Chen, Alex F.

AU - Premkumar, Louis S.

AU - Torry, Donald S.

AU - Lee, Tony J F

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