Endogenous Activation Patterns of Cdc42 GTPase Within Drosophila Embryos

Daichi Kamiyama, Akira Chiba

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Knowing when and where a given protein is activated within intact animals assists in elucidating its in vivo function. With the use of a genetically encoded A-probe (activation bioprobe), we revealed that Cdc42 guanosine triphosphatase (GTPase) remains inactive within Drosophila embryos during the first two-thirds of embryogenesis. Within the central nervous system where Cdc42 activity first becomes up-regulated, individual neurons display patterns restricted to specific subcellular compartments. At both organismal and cellular levels, Cdc42's endogenous activation patterns in the wild type allow predictions of where loss-of-function phenotypes will emerge in cdc42/cdc42 mutants. Genetic tests support the importance of suppressing endogenous Cdc42 activities until needed. Thus, bioprobe-assisted analysis uncovers how ubiquitously expressed signaling proteins control cellular events through continual regulation of their activities within animals.

Original languageEnglish
Pages (from-to)1338-1340
Number of pages3
JournalScience
Volume324
Issue number5932
DOIs
StatePublished - Jun 15 2009

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Guanosine
Biosensing Techniques
Drosophila
Embryonic Structures
Embryonic Development
Proteins
Central Nervous System
Phenotype
Neurons

ASJC Scopus subject areas

  • General

Cite this

Endogenous Activation Patterns of Cdc42 GTPase Within Drosophila Embryos. / Kamiyama, Daichi; Chiba, Akira.

In: Science, Vol. 324, No. 5932, 15.06.2009, p. 1338-1340.

Research output: Contribution to journalArticle

Kamiyama, Daichi ; Chiba, Akira. / Endogenous Activation Patterns of Cdc42 GTPase Within Drosophila Embryos. In: Science. 2009 ; Vol. 324, No. 5932. pp. 1338-1340.
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