Abstract
Knowing when and where a given protein is activated within intact animals assists in elucidating its in vivo function. With the use of a genetically encoded A-probe (activation bioprobe), we revealed that Cdc42 guanosine triphosphatase (GTPase) remains inactive within Drosophila embryos during the first two-thirds of embryogenesis. Within the central nervous system where Cdc42 activity first becomes up-regulated, individual neurons display patterns restricted to specific subcellular compartments. At both organismal and cellular levels, Cdc42's endogenous activation patterns in the wild type allow predictions of where loss-of-function phenotypes will emerge in cdc42/cdc42 mutants. Genetic tests support the importance of suppressing endogenous Cdc42 activities until needed. Thus, bioprobe-assisted analysis uncovers how ubiquitously expressed signaling proteins control cellular events through continual regulation of their activities within animals.
| Original language | English |
|---|---|
| Pages (from-to) | 1338-1340 |
| Number of pages | 3 |
| Journal | Science |
| Volume | 324 |
| Issue number | 5932 |
| DOIs | |
| State | Published - Jun 15 2009 |
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Cite this
Endogenous Activation Patterns of Cdc42 GTPase Within Drosophila Embryos. / Kamiyama, Daichi; Chiba, Akira.
In: Science, Vol. 324, No. 5932, 15.06.2009, p. 1338-1340.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endogenous Activation Patterns of Cdc42 GTPase Within Drosophila Embryos
AU - Kamiyama, Daichi
AU - Chiba, Akira
PY - 2009/6/15
Y1 - 2009/6/15
N2 - Knowing when and where a given protein is activated within intact animals assists in elucidating its in vivo function. With the use of a genetically encoded A-probe (activation bioprobe), we revealed that Cdc42 guanosine triphosphatase (GTPase) remains inactive within Drosophila embryos during the first two-thirds of embryogenesis. Within the central nervous system where Cdc42 activity first becomes up-regulated, individual neurons display patterns restricted to specific subcellular compartments. At both organismal and cellular levels, Cdc42's endogenous activation patterns in the wild type allow predictions of where loss-of-function phenotypes will emerge in cdc42/cdc42 mutants. Genetic tests support the importance of suppressing endogenous Cdc42 activities until needed. Thus, bioprobe-assisted analysis uncovers how ubiquitously expressed signaling proteins control cellular events through continual regulation of their activities within animals.
AB - Knowing when and where a given protein is activated within intact animals assists in elucidating its in vivo function. With the use of a genetically encoded A-probe (activation bioprobe), we revealed that Cdc42 guanosine triphosphatase (GTPase) remains inactive within Drosophila embryos during the first two-thirds of embryogenesis. Within the central nervous system where Cdc42 activity first becomes up-regulated, individual neurons display patterns restricted to specific subcellular compartments. At both organismal and cellular levels, Cdc42's endogenous activation patterns in the wild type allow predictions of where loss-of-function phenotypes will emerge in cdc42/cdc42 mutants. Genetic tests support the importance of suppressing endogenous Cdc42 activities until needed. Thus, bioprobe-assisted analysis uncovers how ubiquitously expressed signaling proteins control cellular events through continual regulation of their activities within animals.
UR - http://www.scopus.com/inward/record.url?scp=66749144802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66749144802&partnerID=8YFLogxK
U2 - 10.1126/science.1170615
DO - 10.1126/science.1170615
M3 - Article
VL - 324
SP - 1338
EP - 1340
JO - Science
JF - Science
SN - 0036-8075
IS - 5932
ER -