End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regeneration

Bu Kyu Lee, Young Min Ju, Jae Gu Cho, John D. Jackson, Sang Jin Lee, Anthony Atala, James J. Yoo

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In cases of complex neuromuscular defects, finding the proximal stump of a transected nerve in order to restore innervation to damaged muscle is often impossible. In this study we investigated whether a neighboring uninjured nerve could serve as a source of innervation of denervated damaged muscle through a biomaterial-based nerve conduit while preserving the uninjured nerve function. Tubular nerve conduits were fabricated by electrospinning a polymer blend consisting of poly(ε-caprolactone) (PCL) and type I collagen. Using a rat model of common peroneal injury, the proximal end of the nerve conduit was connected to the side of the adjacent uninjured tibial branch (TB) of the sciatic nerve after partial axotomy, and the distal end of the conduit was connected to the distal stump of the common peroneal nerve (CPN). The axonal continuity recovered through the nerve conduit at 8 weeks after surgery. Recovery of denervated muscle function was achieved, and simultaneously, the donor muscle, which was innervated by the axotomized TB also recovered at 20 weeks after surgery. Therefore, this end-to-side neurorrhaphy (ETS) technique using the electrospun PCL/collagen conduit appears to be clinically feasible and would be a useful alternative in instances where autologous nerve grafts or an adequate proximal nerve stump is unavailable.

Original languageEnglish
Pages (from-to)9027-9036
Number of pages10
JournalBiomaterials
Volume33
Issue number35
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

Fingerprint

Nerve Regeneration
Peripheral Nerves
Collagen
Muscle
Muscles
Surgery
Axotomy
Peroneal Nerve
Electrospinning
Biocompatible Materials
Sciatic Nerve
Polymer blends
Collagen Type I
Biomaterials
Grafts
Rats
Polymers
Transplants
Recovery
Defects

Keywords

  • End to side neurorrhaphy
  • Nerve guidance channel
  • Nerve repair
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regeneration. / Lee, Bu Kyu; Ju, Young Min; Cho, Jae Gu; Jackson, John D.; Lee, Sang Jin; Atala, Anthony; Yoo, James J.

In: Biomaterials, Vol. 33, No. 35, 01.12.2012, p. 9027-9036.

Research output: Contribution to journalArticle

Lee, Bu Kyu ; Ju, Young Min ; Cho, Jae Gu ; Jackson, John D. ; Lee, Sang Jin ; Atala, Anthony ; Yoo, James J. / End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regeneration. In: Biomaterials. 2012 ; Vol. 33, No. 35. pp. 9027-9036.
@article{22da5cdb429b4e1083d36358cb74958e,
title = "End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regeneration",
abstract = "In cases of complex neuromuscular defects, finding the proximal stump of a transected nerve in order to restore innervation to damaged muscle is often impossible. In this study we investigated whether a neighboring uninjured nerve could serve as a source of innervation of denervated damaged muscle through a biomaterial-based nerve conduit while preserving the uninjured nerve function. Tubular nerve conduits were fabricated by electrospinning a polymer blend consisting of poly(ε-caprolactone) (PCL) and type I collagen. Using a rat model of common peroneal injury, the proximal end of the nerve conduit was connected to the side of the adjacent uninjured tibial branch (TB) of the sciatic nerve after partial axotomy, and the distal end of the conduit was connected to the distal stump of the common peroneal nerve (CPN). The axonal continuity recovered through the nerve conduit at 8 weeks after surgery. Recovery of denervated muscle function was achieved, and simultaneously, the donor muscle, which was innervated by the axotomized TB also recovered at 20 weeks after surgery. Therefore, this end-to-side neurorrhaphy (ETS) technique using the electrospun PCL/collagen conduit appears to be clinically feasible and would be a useful alternative in instances where autologous nerve grafts or an adequate proximal nerve stump is unavailable.",
keywords = "End to side neurorrhaphy, Nerve guidance channel, Nerve repair, Tissue engineering",
author = "Lee, {Bu Kyu} and Ju, {Young Min} and Cho, {Jae Gu} and Jackson, {John D.} and Lee, {Sang Jin} and Anthony Atala and Yoo, {James J.}",
year = "2012",
month = "12",
day = "1",
doi = "10.1016/j.biomaterials.2012.09.008",
language = "English",
volume = "33",
pages = "9027--9036",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "35",

}

TY - JOUR

T1 - End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regeneration

AU - Lee, Bu Kyu

AU - Ju, Young Min

AU - Cho, Jae Gu

AU - Jackson, John D.

AU - Lee, Sang Jin

AU - Atala, Anthony

AU - Yoo, James J.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - In cases of complex neuromuscular defects, finding the proximal stump of a transected nerve in order to restore innervation to damaged muscle is often impossible. In this study we investigated whether a neighboring uninjured nerve could serve as a source of innervation of denervated damaged muscle through a biomaterial-based nerve conduit while preserving the uninjured nerve function. Tubular nerve conduits were fabricated by electrospinning a polymer blend consisting of poly(ε-caprolactone) (PCL) and type I collagen. Using a rat model of common peroneal injury, the proximal end of the nerve conduit was connected to the side of the adjacent uninjured tibial branch (TB) of the sciatic nerve after partial axotomy, and the distal end of the conduit was connected to the distal stump of the common peroneal nerve (CPN). The axonal continuity recovered through the nerve conduit at 8 weeks after surgery. Recovery of denervated muscle function was achieved, and simultaneously, the donor muscle, which was innervated by the axotomized TB also recovered at 20 weeks after surgery. Therefore, this end-to-side neurorrhaphy (ETS) technique using the electrospun PCL/collagen conduit appears to be clinically feasible and would be a useful alternative in instances where autologous nerve grafts or an adequate proximal nerve stump is unavailable.

AB - In cases of complex neuromuscular defects, finding the proximal stump of a transected nerve in order to restore innervation to damaged muscle is often impossible. In this study we investigated whether a neighboring uninjured nerve could serve as a source of innervation of denervated damaged muscle through a biomaterial-based nerve conduit while preserving the uninjured nerve function. Tubular nerve conduits were fabricated by electrospinning a polymer blend consisting of poly(ε-caprolactone) (PCL) and type I collagen. Using a rat model of common peroneal injury, the proximal end of the nerve conduit was connected to the side of the adjacent uninjured tibial branch (TB) of the sciatic nerve after partial axotomy, and the distal end of the conduit was connected to the distal stump of the common peroneal nerve (CPN). The axonal continuity recovered through the nerve conduit at 8 weeks after surgery. Recovery of denervated muscle function was achieved, and simultaneously, the donor muscle, which was innervated by the axotomized TB also recovered at 20 weeks after surgery. Therefore, this end-to-side neurorrhaphy (ETS) technique using the electrospun PCL/collagen conduit appears to be clinically feasible and would be a useful alternative in instances where autologous nerve grafts or an adequate proximal nerve stump is unavailable.

KW - End to side neurorrhaphy

KW - Nerve guidance channel

KW - Nerve repair

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84867143347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867143347&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2012.09.008

DO - 10.1016/j.biomaterials.2012.09.008

M3 - Article

C2 - 22998812

AN - SCOPUS:84867143347

VL - 33

SP - 9027

EP - 9036

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 35

ER -