Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats

Jean Paul Vallée, Mathieu Hauwel, Matthieu Lepetit-Coiffée, Wang Bei, Karin Montet-Abou, Paolo Meda, Stephany Gardier, Prisca Zammaretti, Thomas P. Kraehenbuehl, Francois Herrmann, Jeffrey A. Hubbell, Marisa E. Jaconi

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Pluripotent stem cell-seeded cardiopatches hold promise for in situ regeneration of infarcted hearts. Here, we describe a novel cardiopatch based on bone morphogenetic protein 2-primed cardiac-committed mouse embryonic stem cells, embedded into biodegradable fibrin matrices and engrafted onto infarcted rat hearts. For in vivo tracking of the engrafted cardiac-committed cells, superparamagnetic iron oxide nanoparticles were magnetofected into the cells, thus enabling detection and functional evaluation by high-resolution magnetic resonance imaging. Six weeks after transplantation into infarcted rat hearts, both local (p <.04) and global (p <.015) heart function, as well as the left ventricular dilation (p <.0011), were significantly improved (p <.001) as compared with hearts receiving cardiopatches loaded with iron nanoparticles alone. Histological analysis revealed that the fibrin scaffolds had degraded over time and clusters of myocyte enhancer factor 2-positive cardiac-committed cells had colonized most of the infarcted myocardium, including the fibrotic area. De novo CD31-positive blood vessels were formed in the vicinity of the transplanted cardiopatch. Altogether, our data provide evidence that stem cell-based cardiopatches represent a promising therapeutic strategy to achieve efficient cell implantation and improved global and regional cardiac function after myocardial infarction.

Original languageEnglish (US)
Pages (from-to)248-260
Number of pages13
JournalStem Cells Translational Medicine
Issue number3
StatePublished - 2012


  • Cardiac
  • Embryonic stem cells
  • Stem cell transplantation
  • Tissue regeneration

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)


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