Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats

Jean Paul Vallée, Mathieu Hauwel, Matthieu Lepetit-Coiffée, Wang Bei, Karin Montet-Abou, Paolo Meda, Stephany Gardier, Prisca Zammaretti, Thomas P. Kraehenbuehl, Francois Herrmann, Jeffrey A. Hubbell, Marisa E. Jaconi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Pluripotent stem cell-seeded cardiopatches hold promise for in situ regeneration of infarcted hearts. Here, we describe a novel cardiopatch based on bone morphogenetic protein 2-primed cardiac-committed mouse embryonic stem cells, embedded into biodegradable fibrin matrices and engrafted onto infarcted rat hearts. For in vivo tracking of the engrafted cardiac-committed cells, superparamagnetic iron oxide nanoparticles were magnetofected into the cells, thus enabling detection and functional evaluation by high-resolution magnetic resonance imaging. Six weeks after transplantation into infarcted rat hearts, both local (p <.04) and global (p <.015) heart function, as well as the left ventricular dilation (p <.0011), were significantly improved (p <.001) as compared with hearts receiving cardiopatches loaded with iron nanoparticles alone. Histological analysis revealed that the fibrin scaffolds had degraded over time and clusters of myocyte enhancer factor 2-positive cardiac-committed cells had colonized most of the infarcted myocardium, including the fibrotic area. De novo CD31-positive blood vessels were formed in the vicinity of the transplanted cardiopatch. Altogether, our data provide evidence that stem cell-based cardiopatches represent a promising therapeutic strategy to achieve efficient cell implantation and improved global and regional cardiac function after myocardial infarction.

Original languageEnglish
Pages (from-to)248-260
Number of pages13
JournalStem cells translational medicine
Volume1
Issue number3
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

Fingerprint

Embryonic Stem Cells
Fibrin
Nanoparticles
MEF2 Transcription Factors
Bone Morphogenetic Protein 2
Pluripotent Stem Cells
Blood Vessels
Regeneration
Dilatation
Myocardium
Stem Cells
Iron
Transplantation
Myocardial Infarction
Magnetic Resonance Imaging
Therapeutics

Keywords

  • Cardiac
  • Embryonic stem cells
  • Stem cell transplantation
  • Tissue regeneration

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this

Vallée, J. P., Hauwel, M., Lepetit-Coiffée, M., Bei, W., Montet-Abou, K., Meda, P., ... Jaconi, M. E. (2012). Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats. Stem cells translational medicine, 1(3), 248-260. https://doi.org/10.5966/sctm.2011-0028

Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats. / Vallée, Jean Paul; Hauwel, Mathieu; Lepetit-Coiffée, Matthieu; Bei, Wang; Montet-Abou, Karin; Meda, Paolo; Gardier, Stephany; Zammaretti, Prisca; Kraehenbuehl, Thomas P.; Herrmann, Francois; Hubbell, Jeffrey A.; Jaconi, Marisa E.

In: Stem cells translational medicine, Vol. 1, No. 3, 01.12.2012, p. 248-260.

Research output: Contribution to journalArticle

Vallée, JP, Hauwel, M, Lepetit-Coiffée, M, Bei, W, Montet-Abou, K, Meda, P, Gardier, S, Zammaretti, P, Kraehenbuehl, TP, Herrmann, F, Hubbell, JA & Jaconi, ME 2012, 'Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats', Stem cells translational medicine, vol. 1, no. 3, pp. 248-260. https://doi.org/10.5966/sctm.2011-0028
Vallée JP, Hauwel M, Lepetit-Coiffée M, Bei W, Montet-Abou K, Meda P et al. Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats. Stem cells translational medicine. 2012 Dec 1;1(3):248-260. https://doi.org/10.5966/sctm.2011-0028
Vallée, Jean Paul ; Hauwel, Mathieu ; Lepetit-Coiffée, Matthieu ; Bei, Wang ; Montet-Abou, Karin ; Meda, Paolo ; Gardier, Stephany ; Zammaretti, Prisca ; Kraehenbuehl, Thomas P. ; Herrmann, Francois ; Hubbell, Jeffrey A. ; Jaconi, Marisa E. / Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats. In: Stem cells translational medicine. 2012 ; Vol. 1, No. 3. pp. 248-260.
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