Embryonic neurons transplanted into the tibial nerve reinnervate muscle and reduce atrophy but NCAM expression persists

Robert M. Grumbles, Vania W. Almeida, Christine K Thomas

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: The aim of this study was to use the glycogen depletion technique to determine whether reinnervated muscle fibers could be distinguished from denervated muscle fibers by their size or by neural cell adhesion molecule (NCAM) expression. Methods: Medial gastrocnemius muscles of five adult Fischer rats were reinnervated from embryonic neurons transplanted into the distal stump of the tibial nerve. Ten weeks later, the transplants were stimulated repeatedly to deplete reinnervated muscle fibers of glycogen. Areas of reinnervated (glycogen-depleted) muscle fibers were measured and assessed for NCAM expression. The areas of muscle fibers from reinnervated, denervated (n=5) and unoperated control muscles (n=5) were compared. Results: Mean reinnervated muscle fiber area was significantly larger than the mean for denervated fibers (mean ± SE: 40 ± 6 and 10 ± 1% of unoperated control fibers, respectively). NCAM was expressed in 55 ± 7% of reinnervated fibers (mean ± SE; range: 42-77%). The mean areas of reinnervated fibers that did or did not express NCAM were similar. NCAM was only expressed in some fibers in completely denervated muscles. Discussion: Our data show that NCAM expression does not differentiate muscle denervation or reinnervation. Quantifying the area of large fibers did distinguish reinnervated muscle fibers from denervated fibers and showed that reinnervation of muscle from neurons placed in peripheral nerve is a strategy to rescue muscle from atrophy.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalNeurological Research
Volume30
Issue number2
DOIs
StatePublished - Mar 1 2008

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Neural Cell Adhesion Molecules
Tibial Nerve
Muscular Atrophy
Neurons
Muscles
Glycogen
Muscle Denervation
Inbred F344 Rats
Peripheral Nerves
Skeletal Muscle
Transplants

Keywords

  • Denervated muscle
  • Functional reinnervation
  • Motoneuron transplantation
  • Neural cell adhesion molecule

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Embryonic neurons transplanted into the tibial nerve reinnervate muscle and reduce atrophy but NCAM expression persists. / Grumbles, Robert M.; Almeida, Vania W.; Thomas, Christine K.

In: Neurological Research, Vol. 30, No. 2, 01.03.2008, p. 183-189.

Research output: Contribution to journalArticle

Grumbles, Robert M. ; Almeida, Vania W. ; Thomas, Christine K. / Embryonic neurons transplanted into the tibial nerve reinnervate muscle and reduce atrophy but NCAM expression persists. In: Neurological Research. 2008 ; Vol. 30, No. 2. pp. 183-189.
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