Embryonic gene expression among pollutant resistant and sensitive Fundulus heteroclitus populations

Goran Bozinovic, Marjorie F Oleksiak

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Changes in gene expression, coupled with biochemical, physiological, and behavioral alterations, play a critical role in adaptation to environmental stress. Our goal was to explore ways natural populations may have adapted to local, polluted environments. We took advantage of natural populations of Fundulus heteroclitus, one of the few studied fish species in North America that has established resistant populations in highly contaminated urban estuaries. We analyzed morphology, physiology, and gene expression of developing F. heteroclitus embryos during late organogenesis (stage 31); these embryos were from both resistant and sensitive populations and were raised in a common, unpolluted environment. While cardiac heart rates show significant differences between embryos of parents from clean and heavily contaminated Superfund sites, time-to-stage, embryo morphology, and gene expression profile analyses do not differ significantly between untreated embryos from resistant and sensitive populations. Further evaluation that includes tissue-specific approaches in gene expression analysis and larger sample sizes may be necessary to highlight important phenotypes associated with mechanisms of sensitivity and resistance among natural F. heteroclitus embryo populations. Alternatively, population differences may be masked by developmental canalization, and biologically important differences between sensitive and resistant embryos may only manifest with exposure (e.g., be dependent on gene by environment interactions).

Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalAquatic Toxicology
Volume98
Issue number3
DOIs
StatePublished - Jul 1 2010

Fingerprint

Fundulidae
Fundulus heteroclitus
gene expression
embryo
embryo (animal)
Embryonic Structures
pollutants
Gene Expression
pollutant
Population
Vulnerable Populations
Estuaries
Gene-Environment Interaction
Superfund
Organogenesis
environmental stress
North America
organogenesis
Transcriptome
Innate Immunity

Keywords

  • Developmental stages
  • Embryo gene expression
  • Fundulus heteroclitus
  • Microarrays

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Embryonic gene expression among pollutant resistant and sensitive Fundulus heteroclitus populations. / Bozinovic, Goran; Oleksiak, Marjorie F.

In: Aquatic Toxicology, Vol. 98, No. 3, 01.07.2010, p. 221-229.

Research output: Contribution to journalArticle

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