TY - JOUR
T1 - Embryonic cerebral cortex cells retain CNS phenotypes after transplantation into peripheral nerve
AU - Baez, Juan Carlos
AU - Gajavelli, Shyam
AU - Thomas, Christine K.
AU - Grumbles, Robert M.
AU - Aparicio, Beatriz
AU - Byer, David
AU - Tsoulfas, Pantelis
PY - 2004/10/1
Y1 - 2004/10/1
N2 - Differentiation of stem cells depends on environmental cues. In this study, acutely dissociated or expanded cells derived from embryonic day 14 (E14) rat cerebral cortex were transplanted into the distal tibial nerve stump of adult Fischer rats to determine whether a peripheral nervous system (PNS) environment would influence cell differentiation. Acutely dissociated cells, which included neural precursors and post-mitotic neurons, were transplanted immediately after harvest. Expanded cortical cells were transplanted after 8 days of culture with fibroblast growth factor-2 (FGF-2), a process that yields a population of neural stem cells and/or neural precursors. After 2 or 10 weeks in peripheral nerve, the majority of the transplanted cells was astrocytes, as judged from glial fibrillary acid protein (GFAP) expression. Only acutely dissociated transplants had cells that exhibited neuronal phenotypes. Those neurons present in transplants at 10 weeks stained positive for glutamate decarboxylase and did not reinnervate muscle. Maintenance of this cortical phenotype in peripheral nerve suggests that it is necessary to transplant cells with neural phenotypes appropriate for muscle to restore its function.
AB - Differentiation of stem cells depends on environmental cues. In this study, acutely dissociated or expanded cells derived from embryonic day 14 (E14) rat cerebral cortex were transplanted into the distal tibial nerve stump of adult Fischer rats to determine whether a peripheral nervous system (PNS) environment would influence cell differentiation. Acutely dissociated cells, which included neural precursors and post-mitotic neurons, were transplanted immediately after harvest. Expanded cortical cells were transplanted after 8 days of culture with fibroblast growth factor-2 (FGF-2), a process that yields a population of neural stem cells and/or neural precursors. After 2 or 10 weeks in peripheral nerve, the majority of the transplanted cells was astrocytes, as judged from glial fibrillary acid protein (GFAP) expression. Only acutely dissociated transplants had cells that exhibited neuronal phenotypes. Those neurons present in transplants at 10 weeks stained positive for glutamate decarboxylase and did not reinnervate muscle. Maintenance of this cortical phenotype in peripheral nerve suggests that it is necessary to transplant cells with neural phenotypes appropriate for muscle to restore its function.
KW - Central nervous system
KW - Embryonic cerebral cortex
KW - Peripheral nervous system
UR - http://www.scopus.com/inward/record.url?scp=4544289050&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4544289050&partnerID=8YFLogxK
U2 - 10.1016/j.expneurol.2004.06.013
DO - 10.1016/j.expneurol.2004.06.013
M3 - Article
C2 - 15380492
AN - SCOPUS:4544289050
VL - 189
SP - 422
EP - 425
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 2
ER -