Embryonic cerebral cortex cells retain CNS phenotypes after transplantation into peripheral nerve

Juan Carlos Baez, Shyam Gajavelli, Christine K Thomas, Robert M. Grumbles, Beatriz Aparicio, David Byer, Pantelis Tsoulfas

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Differentiation of stem cells depends on environmental cues. In this study, acutely dissociated or expanded cells derived from embryonic day 14 (E14) rat cerebral cortex were transplanted into the distal tibial nerve stump of adult Fischer rats to determine whether a peripheral nervous system (PNS) environment would influence cell differentiation. Acutely dissociated cells, which included neural precursors and post-mitotic neurons, were transplanted immediately after harvest. Expanded cortical cells were transplanted after 8 days of culture with fibroblast growth factor-2 (FGF-2), a process that yields a population of neural stem cells and/or neural precursors. After 2 or 10 weeks in peripheral nerve, the majority of the transplanted cells was astrocytes, as judged from glial fibrillary acid protein (GFAP) expression. Only acutely dissociated transplants had cells that exhibited neuronal phenotypes. Those neurons present in transplants at 10 weeks stained positive for glutamate decarboxylase and did not reinnervate muscle. Maintenance of this cortical phenotype in peripheral nerve suggests that it is necessary to transplant cells with neural phenotypes appropriate for muscle to restore its function.

Original languageEnglish
Pages (from-to)422-425
Number of pages4
JournalExperimental Neurology
Volume189
Issue number2
DOIs
StatePublished - Oct 1 2004

Fingerprint

Peripheral Nerves
Cerebral Cortex
Transplantation
Phenotype
Transplants
Neurons
Muscles
Tibial Nerve
Glutamate Decarboxylase
Neural Stem Cells
Glial Fibrillary Acidic Protein
Inbred F344 Rats
Peripheral Nervous System
Fibroblast Growth Factor 2
Astrocytes
Cues
Cell Differentiation
Stem Cells
Maintenance
Population

Keywords

  • Central nervous system
  • Embryonic cerebral cortex
  • Peripheral nervous system

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Embryonic cerebral cortex cells retain CNS phenotypes after transplantation into peripheral nerve. / Baez, Juan Carlos; Gajavelli, Shyam; Thomas, Christine K; Grumbles, Robert M.; Aparicio, Beatriz; Byer, David; Tsoulfas, Pantelis.

In: Experimental Neurology, Vol. 189, No. 2, 01.10.2004, p. 422-425.

Research output: Contribution to journalArticle

Baez, Juan Carlos ; Gajavelli, Shyam ; Thomas, Christine K ; Grumbles, Robert M. ; Aparicio, Beatriz ; Byer, David ; Tsoulfas, Pantelis. / Embryonic cerebral cortex cells retain CNS phenotypes after transplantation into peripheral nerve. In: Experimental Neurology. 2004 ; Vol. 189, No. 2. pp. 422-425.
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