Embryoid body formation of human amniotic fluid stem cells depends on mTOR

A. Valli, M. Rosner, C. Fuchs, N. Siegel, C. E. Bishop, H. Dolznig, U. Mädel, W. Feichtinger, A. Atala, M. Hengstschläger

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Human amniotic fluid stem cells (hAFSCs) harbor high proliferative capacity and high differentiation potential and do not raise the ethical concerns associated with human embryonic stem cells. The formation of three-dimensional aggregates known as embryoid bodies (EBs) is the principal step in the differentiation of pluripotent embryonic stem cells. Using c-Kit-positive hAFSC lines, we show here that these stem cells harbor the potential to form EBs. As part of the two kinase complexes, mTORC1 and mTORC2, mammalian target of rapamycin (mTOR) is the key component of an important signaling pathway, which is involved in the regulation of cell proliferation, growth, tumor development and differentiation. Blocking intracellular mTOR activity through the inhibitor rapamycin or through specific small interfering RNA approaches revealed hAFSC EB formation to depend on mTORC1 and mTORC2. These findings demonstrate hAFSCs to be a new and powerful biological system to recapitulate the three-dimensional and tissue level contexts of in vivo development and identify the mTOR pathway to be essential for this process.

Original languageEnglish
Pages (from-to)966-977
Number of pages12
JournalOncogene
Volume29
Issue number7
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Fingerprint

Embryoid Bodies
Sirolimus
Amniotic Fluid
Stem Cells
Pluripotent Stem Cells
Embryonic Stem Cells
Growth and Development
Human Body
Small Interfering RNA
Phosphotransferases
Cell Proliferation
Cell Line
Neoplasms

Keywords

  • Amniotic fluid
  • EB
  • MTOR
  • Stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Valli, A., Rosner, M., Fuchs, C., Siegel, N., Bishop, C. E., Dolznig, H., ... Hengstschläger, M. (2010). Embryoid body formation of human amniotic fluid stem cells depends on mTOR. Oncogene, 29(7), 966-977. https://doi.org/10.1038/onc.2009.405

Embryoid body formation of human amniotic fluid stem cells depends on mTOR. / Valli, A.; Rosner, M.; Fuchs, C.; Siegel, N.; Bishop, C. E.; Dolznig, H.; Mädel, U.; Feichtinger, W.; Atala, A.; Hengstschläger, M.

In: Oncogene, Vol. 29, No. 7, 01.02.2010, p. 966-977.

Research output: Contribution to journalArticle

Valli, A, Rosner, M, Fuchs, C, Siegel, N, Bishop, CE, Dolznig, H, Mädel, U, Feichtinger, W, Atala, A & Hengstschläger, M 2010, 'Embryoid body formation of human amniotic fluid stem cells depends on mTOR', Oncogene, vol. 29, no. 7, pp. 966-977. https://doi.org/10.1038/onc.2009.405
Valli A, Rosner M, Fuchs C, Siegel N, Bishop CE, Dolznig H et al. Embryoid body formation of human amniotic fluid stem cells depends on mTOR. Oncogene. 2010 Feb 1;29(7):966-977. https://doi.org/10.1038/onc.2009.405
Valli, A. ; Rosner, M. ; Fuchs, C. ; Siegel, N. ; Bishop, C. E. ; Dolznig, H. ; Mädel, U. ; Feichtinger, W. ; Atala, A. ; Hengstschläger, M. / Embryoid body formation of human amniotic fluid stem cells depends on mTOR. In: Oncogene. 2010 ; Vol. 29, No. 7. pp. 966-977.
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