EmaA, a potential virulence determinant of Aggregatibacter actinomycetemcomitans in infective endocarditis

Gaoyan Tang, Todd Kitten, Cindy Munro, George C. Wellman, Keith P. Mintz

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The gram-negative fastidious human oropharyngeal Aggregatibacter actinomycetemcomitans is implicated in the etiology of infective endocarditis. EmaA, an oligomeric coiled-coil adhesin homologous to YadA of Yersinia enterocolitica, was hypothesized to mediate the interaction of A. actinomycetemcomitans with collagen. Collagen, the most abundant protein in human bodies and the main component of extracellular matrix (ECM), predominates in the supporting tissue of cardiac valves. To extend our earlier studies using purified collagen to determine bacterial binding activities, we developed a tissue model using rabbit cardiac valves to investigate the interaction of A. actinomycetemcomitans with native collagen. The resected mitral valves, with or without removal of the endothelium, were incubated with equivalent numbers of the wild type and the isogenic emaA mutant defective in collagen binding. There was no difference in binding between the wild-type and the mutant strains when the endothelium remained intact. However, the emaA mutant was fivefold less effective than the wild-type strain in colonizing the exposed ECM. A 10-fold increase in the binding of the wild-type strain to ECM was observed compared with the intact endothelium. Similar observations were replicated in an in vivo endocarditis rabbit model; the emaA mutant was 10-fold less effective in the initial infection of the traumatized aortic valve. Colocalization studies indicated that A. actinomycetemcomitans bound to type I collagen. A. actinomycetemcomitans preferentially colonized the ECM and, together with the evidence that EmaA interacts with the native collagen, suggested that the adhesin is likely a potential virulence determinant of the bacterium in the initiation of infective endocarditis.

Original languageEnglish (US)
Pages (from-to)2316-2324
Number of pages9
JournalInfection and Immunity
Volume76
Issue number6
DOIs
StatePublished - Jun 1 2008
Externally publishedYes

Fingerprint

Aggregatibacter actinomycetemcomitans
Endocarditis
Virulence
Collagen
Extracellular Matrix
Endothelium
Heart Valves
Rabbits
Yersinia enterocolitica
Collagen Type I
Aortic Valve
Mitral Valve
Human Body
Bacteria
Infection
Proteins

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

EmaA, a potential virulence determinant of Aggregatibacter actinomycetemcomitans in infective endocarditis. / Tang, Gaoyan; Kitten, Todd; Munro, Cindy; Wellman, George C.; Mintz, Keith P.

In: Infection and Immunity, Vol. 76, No. 6, 01.06.2008, p. 2316-2324.

Research output: Contribution to journalArticle

Tang, Gaoyan ; Kitten, Todd ; Munro, Cindy ; Wellman, George C. ; Mintz, Keith P. / EmaA, a potential virulence determinant of Aggregatibacter actinomycetemcomitans in infective endocarditis. In: Infection and Immunity. 2008 ; Vol. 76, No. 6. pp. 2316-2324.
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