Abstract
Although normally stable in growing cells, ribosomal RNAs are degraded under conditions of stress, such as starvation, and in response to misassembled or otherwise defective ribosomes in a process termed RNA quality control. Previously, our laboratory found that large fragments of 16S and 23S rRNA accumulate in strains lacking the processive exoribonucleases RNase II, RNase R, and PNPase, implicating these enzymes in the later steps of rRNA breakdown. Here, we define the pathways of rRNA degradation in the quality control process and during starvation, and show that the essential endoribonuclease, RNase E, is required to make the initial cleavages in both degradative processes. We also present evidence that explains why the exoribonuclease, RNase PH, is required to initiate the degradation of rRNA during starvation. The data presented here provide the first detailed description of rRNA degradation in bacterial cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1163-1171 |
| Number of pages | 9 |
| Journal | RNA |
| Volume | 22 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1 2016 |
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Keywords
- 16S rRNA
- 23S rRNA
- Ribonuclease
- Ribosome
ASJC Scopus subject areas
- Molecular Biology
Cite this
Elucidation of pathways of ribosomal RNA degradation : An essential role for RNase E. / Sulthana, Shaheen; Basturea, Georgeta N.; Deutscher, Murray P.
In: RNA, Vol. 22, No. 8, 01.08.2016, p. 1163-1171.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Elucidation of pathways of ribosomal RNA degradation
T2 - An essential role for RNase E
AU - Sulthana, Shaheen
AU - Basturea, Georgeta N.
AU - Deutscher, Murray P
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Although normally stable in growing cells, ribosomal RNAs are degraded under conditions of stress, such as starvation, and in response to misassembled or otherwise defective ribosomes in a process termed RNA quality control. Previously, our laboratory found that large fragments of 16S and 23S rRNA accumulate in strains lacking the processive exoribonucleases RNase II, RNase R, and PNPase, implicating these enzymes in the later steps of rRNA breakdown. Here, we define the pathways of rRNA degradation in the quality control process and during starvation, and show that the essential endoribonuclease, RNase E, is required to make the initial cleavages in both degradative processes. We also present evidence that explains why the exoribonuclease, RNase PH, is required to initiate the degradation of rRNA during starvation. The data presented here provide the first detailed description of rRNA degradation in bacterial cells.
AB - Although normally stable in growing cells, ribosomal RNAs are degraded under conditions of stress, such as starvation, and in response to misassembled or otherwise defective ribosomes in a process termed RNA quality control. Previously, our laboratory found that large fragments of 16S and 23S rRNA accumulate in strains lacking the processive exoribonucleases RNase II, RNase R, and PNPase, implicating these enzymes in the later steps of rRNA breakdown. Here, we define the pathways of rRNA degradation in the quality control process and during starvation, and show that the essential endoribonuclease, RNase E, is required to make the initial cleavages in both degradative processes. We also present evidence that explains why the exoribonuclease, RNase PH, is required to initiate the degradation of rRNA during starvation. The data presented here provide the first detailed description of rRNA degradation in bacterial cells.
KW - 16S rRNA
KW - 23S rRNA
KW - Ribonuclease
KW - Ribosome
UR - http://www.scopus.com/inward/record.url?scp=84979695508&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84979695508&partnerID=8YFLogxK
U2 - 10.1261/rna.056275.116
DO - 10.1261/rna.056275.116
M3 - Article
C2 - 27298395
AN - SCOPUS:84979695508
VL - 22
SP - 1163
EP - 1171
JO - RNA
JF - RNA
SN - 1355-8382
IS - 8
ER -