ELISA for quantitation of l-selectin shed from leukocytes in vivo

Olivier Spertini, Boris Schleiffenbaum, Cathy White-Owen, Phillip Ruiz, Thomas F. Tedder

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

l-selectin is a cell surface receptor on granulocytes, lymphocytes and monocytes that is responsible for the initial attachment of leukocytes to endothelium. The extracellular domain of l-selectin is proteolytically shed from leukocytes following cellular activation in vitro. The shed form of l-selectin (sl-selectin) is functionally active and at high concentrations can inhibit leukocyte attachment to endothelium. Therefore, an ELISA was developed to quantitate the levels of sl-selectin in biological fluids, biopsy specimens and during recombinant protein production. This simple, quantitative sandwich ELISA uses two monoclonal antibodies directed against the extracellular domain of sl-selectin. The assay has a detection range of 5-1300 ng/ml, is precise and sensitive. The ability of this assay to detect sl-selectin in serum, plasma, and culture supernatant fluid was demonstrated and it was used to quantitate circulating sl-selectin in normal and patient sera. Patients with sepsis and HIV infection showed markedly elevated sl-selectin levels in serum. Thus, the ELISA should prove useful both for laboratory purposes as well as in the diagnostic evaluation of patients with inflammatory diseases.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalJournal of Immunological Methods
Volume156
Issue number1
DOIs
StatePublished - Nov 25 1992

Fingerprint

Selectins
Leukocytes
Enzyme-Linked Immunosorbent Assay
Endothelium
Serum
Cell Surface Receptors
Recombinant Proteins
Granulocytes
HIV Infections
Monocytes
Sepsis
Monoclonal Antibodies
Lymphocytes
Biopsy

Keywords

  • ELISA
  • l-Selectin
  • Leukocyte
  • Receptor
  • Shedding

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

ELISA for quantitation of l-selectin shed from leukocytes in vivo. / Spertini, Olivier; Schleiffenbaum, Boris; White-Owen, Cathy; Ruiz, Phillip; Tedder, Thomas F.

In: Journal of Immunological Methods, Vol. 156, No. 1, 25.11.1992, p. 115-123.

Research output: Contribution to journalArticle

Spertini, O, Schleiffenbaum, B, White-Owen, C, Ruiz, P & Tedder, TF 1992, 'ELISA for quantitation of l-selectin shed from leukocytes in vivo', Journal of Immunological Methods, vol. 156, no. 1, pp. 115-123. https://doi.org/10.1016/0022-1759(92)90017-N
Spertini, Olivier ; Schleiffenbaum, Boris ; White-Owen, Cathy ; Ruiz, Phillip ; Tedder, Thomas F. / ELISA for quantitation of l-selectin shed from leukocytes in vivo. In: Journal of Immunological Methods. 1992 ; Vol. 156, No. 1. pp. 115-123.
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