Elevation of RNase R in response to multiple stress conditions

Chenglu Chen, Murray P Deutscher

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Cells respond to adverse environmental conditions by synthesizing new proteins or elevating the levels of pre-existing ones that are needed to cope with the particular stress situation. We show here that Escherichia coli RNase R, a processive 3′- to 5′-exoribonuclease, is dramatically increased in response to a variety of different stress conditions. Elevation of RNase R activity by as much as 10-fold was observed in response to entry into stationary phase, starvation, and cold shock, and a ∼3-fold increase was seen during growth in minimal medium compared with rich medium. The elevation in RNase R activity was associated primarily with an increase in RNase R protein. RNase R was previously implicated in quality control of rRNA and tRNA and in the decay of mRNAs with extensive secondary structure. Its dramatic increase under multiple stress conditions suggests extensive remodeling of structured RNA in response to the altered environment.

Original languageEnglish
Pages (from-to)34393-34396
Number of pages4
JournalJournal of Biological Chemistry
Volume280
Issue number41
DOIs
StatePublished - Oct 14 2005

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RNA Stability
Starvation
Transfer RNA
Quality Control
Escherichia coli
Quality control
Shock
Proteins
ribonuclease R
RNA
Messenger RNA
Growth
5'-exoribonuclease

ASJC Scopus subject areas

  • Biochemistry

Cite this

Elevation of RNase R in response to multiple stress conditions. / Chen, Chenglu; Deutscher, Murray P.

In: Journal of Biological Chemistry, Vol. 280, No. 41, 14.10.2005, p. 34393-34396.

Research output: Contribution to journalArticle

Chen, Chenglu ; Deutscher, Murray P. / Elevation of RNase R in response to multiple stress conditions. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 41. pp. 34393-34396.
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